Optimized Expression of a Thermostable Xylanase 11 A Gene from Chaetomium thermophilum NIBGE 1 in Escherichia coli

被引：3
作者：
Ghaffar, Abdul [1 ,2 ]
Khan, Sher Afzal [1 ]
Mukhtar, Zahid [1 ]
Latif, Farooq [1 ]
Rajoka, Muhammad Ibrahim [1 ]
机构：
[1] NIBGE, Faisalabad, Pakistan
[2] Univ Agr Faisalabad, Dept Chem Biochem, Faisalabad, Pakistan
来源：
PROTEIN AND PEPTIDE LETTERS | 2009年 / 16卷 / 04期
关键词：
Amplification; expression; E; coli; fungal xylanase; gene cloning; kinetics; characterization; TRICHODERMA-REESEI; SACCHAROMYCES-CEREVISIAE; FUNGAL HOSTS; CLONING; MICROORGANISMS; PROTEINS; ENZYMES; BINDING; XYN2;
D O I：
10.2174/092986609787848126
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The xylanase (Xyn11A) gene (883 bp) was amplified using C. thermophilum DNA as template and cloned into pET-32a (+) and expressed in E. coli BL21 under T-7 promoter. The recombinant xylanase on SDS-PAGE had a molecular mass of 30 kDa. Productivity profiles of xylanase in E. coli recombinant are more than 4-fold of that produced from T. reesei RUTC-30, 5-fold of that produced by the donor and significantly higher than the values reported on other E. coli, and Saccharomyces cerevisiae recombinants. Temperature stability, pH stability, and other kinetic parameters confirmed that the gene product was thermo-stable in alkaline buffer favoring its suitability to bio-bleaching of kraft pulp.
引用
收藏
页码：356 / 362
页数：7
相关论文
共 50 条
  • [41] Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from Bacillus licheniformis in Escherichia coli
    Songsiriritthigul, Chomphunuch
    Buranabanyat, Bancha
    Haltrich, Dietmar
    Yamabhai, Montarop
    MICROBIAL CELL FACTORIES, 2010, 9
  • [42] CLONING OF A NOVEL XYLANASE GENE FROM A NEWLY ISOLATED FUSARIUM SP Q7-31 AND ITS EXPRESSION IN ESCHERICHIA COLI
    Xie, Zhan-Ling
    Gao, Hai-Yan
    Zhang, Qian
    Wang, Huan
    Liu, Ying
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2012, 43 (01) : 405 - 417
  • [43] Cloning, expression and purification of laccase enzyme gene from Bacillus subtilis in Escherichia coli
    Ghasemi, Y.
    Yarahmadi, E.
    Ghoshoon, M. B.
    Dabbagh, F.
    Hajighahramani, N.
    Ebrahimi, N.
    Mobasher, M. A.
    Najafabady, N. Montazeri
    MINERVA BIOTECNOLOGICA, 2014, 26 (04) : 295 - 300
  • [44] Cloning and expression of a gene with phospholipase B activity from Pseudomonas fluorescens in Escherichia coli
    Jiang, Fangyan
    Huang, Shen
    Imadad, Kaleem
    Li, Chun
    BIORESOURCE TECHNOLOGY, 2012, 104 : 518 - 522
  • [45] Cloning the hbs gene from Bacillus subtilis and expression of the HBsu protein in Escherichia coli
    Ghodsi, S.
    Gharavi, S.
    Ghadam, P.
    IRANIAN JOURNAL OF MICROBIOLOGY, 2010, 2 (03) : 152 - 156
  • [46] Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins
    Marlatt, Nicole M.
    Spratt, Donald E.
    Shaw, Gary S.
    PROTEIN EXPRESSION AND PURIFICATION, 2010, 73 (01) : 58 - 64
  • [47] Cloning of a Heat-Stable Chitin Deacetylase Gene from Aspergillus nidulans and its Functional Expression in Escherichia coli
    Wang, Yun
    Song, Jin-Zhu
    Yang, Qian
    Liu, Zhi-Hua
    Huang, Xiao-Mei
    Chen, Yan
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2010, 162 (03) : 843 - 854
  • [48] Thermostable alkaline phosphatase of bacterium Meiothermus tuber:: gene cloning, expression in Escherichia coli, and biochemical characterization of the recombinant protein
    Yurchenko, YV
    Khromov, IS
    Budilov, AV
    Deyev, M
    Sobolev, AY
    MOLECULAR BIOLOGY, 2003, 37 (06) : 841 - 848
  • [49] Cloning and expression of mel gene from Bacillus thuringiensis in Escherichia coli
    Ruan, L
    He, W
    He, J
    Sun, M
    Yu, Z
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2005, 87 (04): : 283 - 288
  • [50] Cloning and expression of mel gene from Bacillus thuringiensis in Escherichia coli
    L. Ruan
    W. He
    J. He
    M. Sun
    Z. Yu
    Antonie van Leeuwenhoek, 2005, 87 : 283 - 288
12345