Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1

被引：36

作者：

Hashimoto, K ^{[1
]}

Saito, M

Matsuoka, H

Iida, K

Iida, H

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

[2] Tokyo Metropolitan Inst Med Sci, Med R&D Ctr, Bunkyo Ku, Tokyo 1138613, Japan

[3] Tokyo Gakugei Univ, Dept Biol, Tokyo 1848501, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2004年 / 45卷 / 04期

关键词：

heterologous yeast expression; Oryza sativa; two-pore channel; voltage-dependent calcium channel;

D O I：

10.1093/pcp/pch053

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca2+ channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the alphal-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca2+ permeation. The K. value for Ca2+, of OsTPC1, 47.5 muM, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca2+ permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.

引用

页码：496 / 500

页数：5

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