Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1

被引：36
|
作者：
Hashimoto, K [1 ]
Saito, M
Matsuoka, H
Iida, K
Iida, H
机构：
[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan
[2] Tokyo Metropolitan Inst Med Sci, Med R&D Ctr, Bunkyo Ku, Tokyo 1138613, Japan
[3] Tokyo Gakugei Univ, Dept Biol, Tokyo 1848501, Japan
来源：
PLANT AND CELL PHYSIOLOGY | 2004年 / 45卷 / 04期
关键词：
heterologous yeast expression; Oryza sativa; two-pore channel; voltage-dependent calcium channel;
D O I：
10.1093/pcp/pch053
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca2+ channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the alphal-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca2+ permeation. The K. value for Ca2+, of OsTPC1, 47.5 muM, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca2+ permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.
引用
收藏
页码：496 / 500
页数：5
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