Saliva pools for screening of human cytomegalovirus using real-time PCR

被引：11

作者：

Fernandes, Claudia ^{[1
]}

Marques, Augusta ^{[1
]}

de Jesus Chasqueira, Maria ^{[1
]}

Braz, Monica Cro ^{[2
]}

Ferreira, Ana Rute ^{[2
]}

Neto, Ana Serrao ^{[2
]}

Mendes, Candida ^{[3
]}

Lito, David ^{[3
]}

Menezes, Maria-Favila ^{[4
]}

Sousa, Maria Jose ^{[4
]}

Paixao, Paulo ^{[1
]}

机构：

[1] NOVA Med Sch, Fac Ciencias Med, CEDOC, Ctr Estudos Doencas Cron, Campo Martires Patria 130, P-1169056 Lisbon, Portugal

[2] Hosp CUF Descobertas, Serv Pediat, Rua Mario Botas, P-1998018 Lisbon, Portugal

[3] Hosp Vila Franca Xira, Serv Pediat, Estr Carlos Lima Costa 2, P-2600009 Vila Franca De Xira, Portugal

[4] Cetr Med Lab Germano Sousa, Rua Cupertino Miranda, P-1600513 Lisbon, Portugal

来源：

EUROPEAN JOURNAL OF PEDIATRICS | 2021年 / 180卷 / 04期

关键词：

HCMV; Newborn; Cost reduction; Pools; POLYMERASE-CHAIN-REACTION; CONGENITAL CMV INFECTION; DIAGNOSIS; URINE; EPIDEMIOLOGY; PREVALENCE; DNA;

D O I：

10.1007/s00431-020-03842-x

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Human cytomegalovirus (HCMV) is the leading congenital infection agent in the world. The importance of screening this infection has been debated, as 10-15% of the asymptomatic newborns with HCMV at birth will present late sequelae. The aim of this study was to test the feasibility of using saliva pools from newborns in a screening program for congenital HCMV infection, in two Portuguese hospitals. The screening was based on the use of pools of 10 saliva samples for detection of viral DNA by real-time PCR. Whenever there was a positive pool, the samples were tested individually, and for each positive sample the result was confirmed with a urine sample collected in the first 2 weeks of life. The study involved 1492 newborns. One hundred and fifty pools were screened, with 14 positive results in saliva, but only 10 were confirmed in urine samples, giving a prevalence of congenital HCMV infection in both hospitals of 0.67% (CI95% 0.36 to 1.23%). Conclusion: The overall prevalence of congenital HCMV infection in both hospitals was 0.67%. The use of saliva pools proved to be effective for the screening of this congenital infection, allowing timely screening and confirmation in a large population, with associated cost reduction.

引用

页码：1067 / 1072

页数：6

共 25 条

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