Pulse front adaptive optics in two-photon microscopy

被引:5
|
作者
Sun, Bangshan [1 ]
Salter, Patrick S. [1 ]
Booth, Martin J. [1 ,2 ]
机构
[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
[2] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 3SR, England
基金
英国工程与自然科学研究理事会;
关键词
FEMTOSECOND LASER-PULSES; FLUORESCENCE MICROSCOPY; LENS SYSTEMS; DISTORTION; ABERRATIONS; INTERFEROMETRY; COMPENSATION; RESOLUTION; IMAGE;
D O I
10.1364/OL.40.004999
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Adaptive optics has been extensively studied for the correction of phase front aberrations in optical systems. In systems using ultrafast lasers, distortions can also exist in the pulse front (contour of constant intensity in space and time), but until now their correction has been mostly unexplored due to technological limitations. In this Letter, we apply newly developed pulse front adaptive optics, for the first time to our knowledge, to practical compensation of a two-photon fluorescence microscope. With adaptive correction of the system-induced pulse front distortion, improvements beyond conventional phase correction are demonstrated. (C) 2015 Optical Society of America
引用
收藏
页码:4999 / 5002
页数:4
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