Monoclonal antibody against H1N1 influenza virus hemagglutinin cross reacts with hnRNPA1 and hnRNPA2/B1

被引：6

作者：

Guo, Chunyan ^{[1
]}

Sun, Lijun ^{[1
]}

Hao, Shuangping ^{[2
]}

Huang, Xiaoyan ^{[1
]}

Hu, Hanyu ^{[3
]}

Liang, Daoyan ^{[1
]}

Feng, Qing ^{[1
]}

Li, Yan ^{[1
]}

Feng, Yangmeng ^{[1
]}

Xie, Xin ^{[4
]}

Hu, Jun ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Shaanxi Prov Res Ctr Cell Immunol Engn & Technol, Cent Lab Shaanxi Prov Peoples Hosp, Affiliated Hosp 3,Med Sch,Key Lab Microbial Infec, 256 Youyi West Rd, Xian 710068, Shaanxi, Peoples R China

[2] Guangshui Tradit Chinese Med Hosp Hubei Prov, Guangshui 432700, Hubei, Peoples R China

[3] Tulane Univ, Sch Publ Hlth & Trop Med, New Orleans, LA 70112 USA

[4] Northwest Univ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, 229 Taibai North Rd, Xian 710069, Shaanxi, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2020年 / 22卷 / 05期

关键词：

influenza virus; cross-reactivity; nervous system disease; glycine-rich domain; MOLECULAR MIMICRY; AUTOANTIBODIES; AUTOIMMUNITY; INFECTION;

D O I：

10.3892/mmr.2020.11494

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Following influenza A vaccination, certain individuals exhibit adverse reactions in the nervous system, which causes a problem with the safety of the influenza A vaccine. However, to the best of our knowledge, the underlying mechanism of this is unknown. The present study revealed that a monoclonal antibody (H1-84mAb) against the H1N1 influenza virus hemagglutinin (HA) protein cross-reacted with an antigen from brain tissue. Total brain tissue protein was immunoprecipitated with this cross-reactive antibody, and mass spectrometry revealed that the bound antigens were heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and hnRNPA2/B1. Subsequently, the two proteins were expressed in bacteria and it was demonstrated that H1-84mAb bound to hnRNPA1 and hnRNPA2/B1. These two proteins were expressed in three segments and the cross-reactivity of H1-84mAb with the glycine (Gly)-rich domains of hnRNPA1 (195aa-320aa) and hnRNPA2/B1 (202aa-349aa) was determined using ELISA blocking experiments. It was concluded that the Gly-rich domains of these two proteins are heterophilic antigens that cross-react with influenza virus HA. The association between the heterophilic antigen Gly-rich domains and the safety of influenza A vaccines remains to be investigated.

引用

页码：3969 / 3975

页数：7

共 26 条

[1] Antibodies to influenza nucleoprotein cross-react with human hypocretin receptor 2 [J].