Structural and genetic studies of the O-antigen of Enterobacter cloacae G2277

被引：5
|
作者：
Perepelov, Andrei V. [1 ]
Wang, Min [2 ,3 ]
Filatov, Andrei V. [1 ]
Guo, Xi [3 ]
Shashkov, Alexander S. [1 ]
Wang, Lei [2 ,3 ]
Knirel, Yuriy A. [1 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA, TEDA Sch Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
[3] Nankai Univ, TEDA, TEDA Coll, Tianjin Key Lab Microbial Funct Genom, Tianjin 300457, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2014年 / 387卷
基金：
中国国家自然科学基金; 俄罗斯基础研究基金会;
关键词：
Enterobacter cloacae; Lipopolysaccharide; O-Polysaccharide; O-Antigen gene cluster; Bacterial polysaccharide structure; CLUSTER; LIPOPOLYSACCHARIDE; POLYSACCHARIDES; IDENTIFICATION; BIOSYNTHESIS; STRAINS;
D O I：
10.1016/j.carres.2014.01.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2277 and studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established, where a galacturonic acid (GalA) residue is mono-O-acetylated at position either 2 or 3: -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 4)-alpha-D-GalpA-(1 -> 3)-alpha-D-GalpNAc-(1 -> 2/3 vertical bar OAc similar to 30/60 % The O-antigen gene cluster of E. cloacae G2277 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：10 / 13
页数：4
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