cDNA cloning of ovine pulmonary SP-A, SP-B, and SP-C: isolation of two different sequences for SP-B

被引:6
|
作者
Pietschmann, SM [1 ]
Pison, U [1 ]
机构
[1] Humboldt Univ, Fac Med, Dept Anesthesiol & Intens Care Med, Klinikum Virchow,Klin Anaesthesiol & Operat Inten, D-13353 Berlin, Germany
关键词
pulmonary surfactant; surfactant apoproteins; deletion; ovine protein sequences; messenger ribonucleic acid; complementary deoxyribonucleic acid;
D O I
10.1152/ajplung.2000.278.4.L765
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Pulmonary surfactant promotes alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. The three surfactant proteins SP-A, SP-B, and SP-C contribute to dynamic surface properties involved during respiration. We have cloned and sequenced the complete cDNAs for ovine SP-A and SP-C and two distinct forms of ovine SP-B cDNAs. The nucleotide sequence of ovine SP-A cDNA consists of 1,901 bp and encodes a protein of 248 amino acids. Ovine SP-C cDNA contains 809 bp, predicting a protein of 190 amino acids. Ovine SP-B is encoded by two mRNA species, which differ by a 69-bp in-frame deletion in the region coding for the active airway protein. The larger SP-B cDNA comprises 1,660 bp, encoding a putative protein of 374 amino acids. With the sequences reported, a more complete analysis of surfactant regulation and the determination of their physiological function in vivo will be enabled.
引用
收藏
页码:L765 / L778
页数:14
相关论文
共 50 条
  • [31] Frequency of SP-B and SP-A1 gene polymorphisms in the acute respiratory distress syndrome (ARDS)
    Max, M
    Pison, U
    Floros, J
    ACP-APPLIED CARDIOPULMONARY PATHOPHYSIOLOGY, 1996, 6 (02): : 111 - 118
  • [32] Environmental Pollutant Ozone Causes Damage to Lung Surfactant Protein B (SP-B)
    Hemming, Joanna M.
    Hughes, Brian R.
    Rennie, Adrian R.
    Tomas, Salvador
    Campbell, Richard A.
    Hughes, Arwel V.
    Arnold, Thomas
    Botchway, Stanley W.
    Thompson, Katherine C.
    BIOCHEMISTRY, 2015, 54 (33) : 5185 - 5197
  • [33] Role of the N-Terminal Seven Residues of Surfactant Protein B (SP-B)
    Sharifahmadian, Mahzad
    Sarker, Muzaddid
    Palleboina, Dharamaraju
    Waring, Alan J.
    Walther, Frans J.
    Morrow, Michael R.
    Booth, Valerie
    PLOS ONE, 2013, 8 (09):
  • [34] HYDROPHOBIC SURFACTANT-ASSOCIATED POLYPEPTIDES - SP-C IS A LIPOPEPTIDE WITH 2 PALMITOYLATED CYSTEINE RESIDUES, WHEREAS SP-B LACKS COVALENTLY LINKED FATTY ACYL-GROUPS
    CURSTEDT, T
    JOHANSSON, J
    PERSSON, P
    EKLUND, A
    ROBERTSON, B
    LOWENADLER, B
    JORNVALL, H
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) : 2985 - 2989
  • [35] Nanocarrier Lipid Composition Modulates the Impact of Pulmonary Surfactant Protein B (SP-B) on Cellular Delivery of siRNA
    Guagliardo, Roberta
    Merckx, Pieterjan
    Zamborlin, Agata
    De Backer, Lynn
    Echaide, Mercedes
    Perez-Gil, Jesus
    De Smedt, Stefaan C.
    Raemdonck, Koen
    PHARMACEUTICS, 2019, 11 (09)
  • [36] Nicotine stimulates branching and expression of SP-A and SP-C mRNAs in embryonic mouse lung culture
    Wuenschell, CW
    Zhao, JS
    Tefft, JD
    Warburton, D
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1998, 274 (01) : L165 - L170
  • [37] Structure–function correlations of pulmonary surfactant protein SP-B and the saposin-like family of proteins
    Bárbara Olmeda
    Begoña García-Álvarez
    Jesús Pérez-Gil
    European Biophysics Journal, 2013, 42 : 209 - 222
  • [38] Effects of oleic acid on SP-B expression and release in A549 cells
    Zhou, D. -J.
    Chen, Y.
    Zhang, X. -J.
    Ma, C.
    Qiu, J.
    Zhou, J. -H.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2015, 19 (18) : 3438 - 3443
  • [39] New Surfactant with SP-B and C Analogs Gives Survival Benefit after Inactivation in Preterm Lambs
    Seehase, Matthias
    Collins, Jennifer J. P.
    Kuypers, Elke
    Jellema, Reint K.
    Ophelders, Daan R. M. G.
    Ospina, Olga L.
    Perez-Gil, J.
    Bianco, Federico
    Garzia, Raffaella
    Razzetti, Roberta
    Kramer, Boris W.
    PLOS ONE, 2012, 7 (10):
  • [40] Regulation of mouse SP-B gene promoter by AP-1 family members
    Sever-Chroneos, Z
    Bachurski, CJ
    Yan, G
    Whitsett, JA
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1999, 277 (01) : L79 - L88