Pulmonary surfactant promotes alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. The three surfactant proteins SP-A, SP-B, and SP-C contribute to dynamic surface properties involved during respiration. We have cloned and sequenced the complete cDNAs for ovine SP-A and SP-C and two distinct forms of ovine SP-B cDNAs. The nucleotide sequence of ovine SP-A cDNA consists of 1,901 bp and encodes a protein of 248 amino acids. Ovine SP-C cDNA contains 809 bp, predicting a protein of 190 amino acids. Ovine SP-B is encoded by two mRNA species, which differ by a 69-bp in-frame deletion in the region coding for the active airway protein. The larger SP-B cDNA comprises 1,660 bp, encoding a putative protein of 374 amino acids. With the sequences reported, a more complete analysis of surfactant regulation and the determination of their physiological function in vivo will be enabled.
机构:
Univ Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Univ Complutense Madrid, Res Inst, Hosp Octubre 12, Madrid, SpainUniv Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Martinez-Calle, Marta
Alonso, Alejandro
论文数: 0引用数: 0
h-index: 0
机构:
Univ Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Univ Complutense Madrid, Res Inst, Hosp Octubre 12, Madrid, SpainUniv Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Alonso, Alejandro
Perez-Gil, Jesus
论文数: 0引用数: 0
h-index: 0
机构:
Univ Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Univ Complutense Madrid, Res Inst, Hosp Octubre 12, Madrid, SpainUniv Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Perez-Gil, Jesus
Olmeda, Barbara
论文数: 0引用数: 0
h-index: 0
机构:
Univ Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain
Univ Complutense Madrid, Res Inst, Hosp Octubre 12, Madrid, SpainUniv Complutense Madrid, Fac Biol, Dept Biochem, Jose Antonio Novais 12, Madrid 28040, Spain