Characterization of liver specific promoters in a foamy viral vector pMD09

被引：4

作者：

Singh, A. K. ^{[1
]}

Weber, C. ^{[2
]}

Varshney, A. ^{[1
]}

Gupta, S. ^{[1
]}

Kazim, S. N. ^{[3
]}

Sanal, M. G. ^{[1
]}

Rethwilm, A. ^{[2
]}

Sarin, S. K. ^{[4
]}

机构：

[1] Inst Liver & Biliary Sci, Dept Mol & Cellular Med, New Delhi, India

[2] Univ Wurzburg, Inst Virol & Immunbiol, Versbacher Str 7, D-97078 Wurzburg, Germany

[3] Jamia Millia Islamia, Hepatitis Res Lab, Ctr Interdisciplinary Res Basic Sci, New Delhi, India

[4] Inst Liver & Biliary Sci, Dept Hepatol, New Delhi, India

来源：

ACTA VIROLOGICA | 2019年 / 63卷 / 02期

关键词：

foamy virus; gene therapy; liver; albumin; transthyretin promoter; HBV promoter; GENE-THERAPY; VIRUS VECTORS;

D O I：

10.4149/av_2019_207

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Foamy viruses (FVs) or spumaviruses are retroviruses that are explored as vectors for gene therapy. The good feature of foamy viruses is its broad tropism; however, their infections result in non-targeted gene expression. Here, we attempted to design the liver targeted viral gene delivery by employing liver specific gene promoters like albumin (ALB), transthyretin (TTR) and hepatitis B virus (HBV) promoters. We compared the relative gene expression of liver specific promoters versus the U3 promoter in liver cell line (HepG2) and non-liver cell lines: human fibrosarcoma cell line (HT1080), baby hamster kidney cell line (BHK), human embryonic kidney cell line (HEK 293T) and cervical cancer cell line (HeLa). We have found that the promoter exchange didn't affect viral assembly. The ability to drive gene expression was best with TTR promoter which was followed by HBV and ALB promoter. The use of TTR, HBV and ALB promoters are helpful in achieving liver specific gene expression.

引用

页码：162 / +

页数：10