Thiol-Epoxy "Click" Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs

被引：47
作者：
Cong Truc Huynh [1 ]
Liu, Fangze [1 ]
Cheng, Yuxuan [1 ]
Coughlin, Katherine A. [2 ]
Alsberg, Eben [1 ,3 ]
机构：
[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
[2] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA
[3] Case Western Reserve Univ, Dept Orthopaed Surg, Cleveland, OH 44106 USA
来源：
ACS APPLIED MATERIALS & INTERFACES | 2018年 / 10卷 / 31期
基金：
美国国家卫生研究院;
关键词：
biomaterial; click chemistry; hydrogel; RNAi; stem cell differentiation; tissue regeneration; MESENCHYMAL STEM-CELLS; RADIOPAQUE EMBOLIC HYDROGELS; POTENTIAL APPLICATION; BIODEGRADABLE HYDROGELS; ARTICULAR-CARTILAGE; SUSTAINED DELIVERY; BLOCK-COPOLYMER; DIFFERENTIATION; RNA; POLYMERIZATION;
D O I：
10.1021/acsami.8b07167
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Thiol-epoxy "click" chemistry is employed for the first time to engineer a new cytocompatible PEG-based hydrogel system in aqueous media with the ability to encapsulate human mesenchymal stem cells (hMSCs) and control their fate for tissue regeneration. Cells were easily encapsulated into the hydrogels and exhibited high cell viability over 4 weeks of culture regardless of the presence of siRNA, complexed with polyethylenimine (PEI) in the form of siRNA/PEI nanocomplexes, indicating the biocompatibility of the developed hydrogel. Loading pro-osteogenic siNoggin in the hydrogel significantly enhanced the osteogenesis of encapsulated hMSCs, demonstrating the potential application of this system in tissue engineering.
引用
收藏
页码：25936 / 25942
页数：7
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