A cleavable amino-thiol linker for reversible linking of amines to DNA

被引：13

作者：

Hojfeldt, Jonas W.

Blakskjær, Peter

Gothelf, Kurt V.

机构：

[1] Univ Aarhus, Dept Chem, Ctr Catalysis & Interdisciplinary Nanosci Ctr, iNANO, DK-8000 Aarhus C, Denmark

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[3] Vipergen, DK-2100 Copenhagen OE, Denmark

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2006年 / 71卷 / 25期

关键词：

D O I：

10.1021/jo0619736

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A cleavable heterobifunctional cross-linker for the reversible conjugation of amines to thiol-modified DNA has been developed and tested. The succinimidyl 2-(vinylsulfonyl)-ethyl carbonate (SVEC) was prepared in three steps and tested for its ability to react with amines and thiols. The linker was efficient for binding leucine to a thiol-modified DNA sequence and for releasing the amino acid at pH 11.8.

引用

页码：9556 / 9559

页数：4

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