Molecular characterization of a new member of the 16SrV group of phytoplasma associated with Bischofia polycarpa (Levl.) Airy Shaw witches' -broom disease in China by a multiple gene-based analysis

被引:8
作者
Lai, F. [1 ]
Song, C. S. [1 ]
Ren, Z. G. [1 ]
Lin, C. L. [1 ]
Xu, Q. C. [1 ]
Li, Y. [1 ]
Piao, C. G. [1 ]
Yu, S. S. [1 ]
Guo, M. W. [1 ]
Tian, G. Z. [1 ]
机构
[1] Chinese Acad Forestry, Key Lab Forest Protect, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Beijing, Peoples R China
基金
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词
Bischofia polycarpa (Levl.) Airy Shaw witches'-broom (BiWB); Phytoplasma; 16S rRNA gene; Vitual RFLP; Classification; Phylogeny; RIBOSOMAL-RNA GENES; FULL-LENGTH DNA; SEQUENCE HETEROGENEITY; FLAVESCENCE-DOREE; ELM YELLOWS; MYCOPLASMALIKE ORGANISM; RFLP ANALYSIS; CLASSIFICATION; DIFFERENTIATION; GRAPEVINE;
D O I
10.1007/s13313-014-0298-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Bischofia polycarpa (Levl.) Airy Shaw witches'-broom disease is widespread in southern China. Infected trees show symptoms of severely proliferating shoots, reduced leaf size, yellowing and reddening of leaves, and internode shortening. We detected the phytoplasma in diseased tissues by 4',6-diamidino-2-phenylindole-HCl fluorescence microscopy and polymerase chain reaction using the universal primers P1/P7. We conducted sequence similarity analyses based on 16S rRNA and 23S rRNA genes, the spacer region between the 16S and 23S rRNA genes (ISR), the partial ribosomal protein-encoding operon (rp), and the elongation factor Tu (tuf) gene of the BiWB phytoplasma. These analyses unanimously showed that the BiWB phytoplasma belongs to the elm yellows group (the 16SrV group). Phylogenetic and computer-simulated restriction fragment length polymorphism analyses using the nearly full-length 16S rRNA gene sequence demonstrated that the BiWB phytoplasma is more closely related to the subgroup 16SrV-B than to the other subgroups in 16SrV. It may represent a novel subgroup, designed as 16SrV-H, which is distinct from other subgroups of phytoplasmas in the 16SrV group.
引用
收藏
页码:557 / 569
页数:13
相关论文
共 57 条
[1]   Detection of a phytoplasma associated with grapevine Flavescence doree in Clematis vitalba [J].
Angelini, E ;
Squizzato, F ;
Lucchetta, G ;
Borgo, M .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2004, 110 (02) :193-201
[2]   Multilocus sequence typing confirms the close genetic interrelatedness of three distinct flavescence doree phytoplasma strain clusters and group 16SrV phytoplasmas infecting grapevine and alder in Europe [J].
Arnaud, Guillaume ;
Malembic-Maher, Sylvie ;
Salar, Pascal ;
Bonnet, Patrick ;
Maixner, Michael ;
Marcone, Carmine ;
Boudon-Padieu, Elisabeth ;
Foissac, Xavier .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (12) :4001-4010
[3]   Diversity and geographical distribution of phytoplasmas infecting China-tree in Argentina [J].
Arneodo, J. D. ;
Marini, D. C. ;
Galdeano, E. ;
Meneguzzi, N. ;
Bacci, M., Jr. ;
Domecq, C. ;
Nome, S. F. ;
Conci, L. R. .
JOURNAL OF PHYTOPATHOLOGY, 2007, 155 (02) :70-75
[4]   Chromosomal organization and nucleotide sequence of the genes coding for the elongation factors G and Tu of the apple proliferation phytoplasma [J].
Berg, M ;
Seemüller, E .
GENE, 1999, 226 (01) :103-109
[5]  
Brenner D.J., 2005, BERGEYS MANUAL SYSTE, P587, DOI 10.1007/0-387-28022-7_13
[6]  
Cai Hong Cai Hong, 2005, Acta Phytopathologica Sinica, V35, P19
[7]  
Chen YX, 1986, J NANJING AGR U, V2, P131
[8]  
Contaldo N, 2012, PHYTOPATHOL MEDITERR, V51, P607
[9]   Detection and differentiation of grapevine yellows phytoplasmas belonging to the elm yellows group and to the stolbur subgroup by PCR amplification of non-ribosomal DNA [J].
Daire, X ;
Clair, D ;
Reinert, W ;
BoudonPadieu, E .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1997, 103 (06) :507-514
[10]   Revised subgroup classification of group 16SrV phytoplasmas and placement of flavescence doree-associated phytoplasmas in two distinct subgroups [J].
Davis, RE ;
Dally, EL .
PLANT DISEASE, 2001, 85 (07) :790-797