Distribution of sialoglycoconjugates in the rat cerebellum and its change with aging

被引：13
作者：
Sasaki, T
Akimoto, Y
Sato, Y
Kawakami, H
Hirano, H
Endo, T
机构：
[1] Tokyo Metropolitan Inst Gerontol, Dept Glycobiol, Itabashi Ku, Tokyo 1730015, Japan
[2] Kyorin Univ, Sch Med, Dept Anat, Tokyo, Japan
[3] Nittai Jusei Med Coll Judo Therapeut, Tokyo, Japan
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2002年 / 50卷 / 09期
关键词：
sialoglycoconjugate; cerebellum; aging; granular layer; Purkinje cell;
D O I：
10.1177/002215540205000904
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We examined the distribution of sialoglycoconjugates in the cerebellum of 9-week-old and 30-month-old rats using light microscopy and electron microscopy in combination with two lectins, Maackia amurensis lectin (MAL) for Siaalpha2-3Gal and Sambucus sieboldiana agglutinin (SSA) for Siaalpha2-6Gal. Each lectin showed characteristic staining patterns. In young adult rats, MAL stained a strongly granular layer, a weakly molecular layer, and the medullary lamina, while SSA more strongly stained the medullary lamina than the molecular and granular layers. After aging, different staining patterns were obtained. Intense SSA reactivity was observed in the granular layer and intense MAL reactivity was observed in the medullary lamina of the aged groups. The reactivity of Purkinje cells with MAL was downregulated in the aged rats. These results indicated that Siaalpha2-3Gal and Siaalpha2-6Gal were expressed in distinct regions of the rat cerebellum and that their expression patterns changed in the aged brain.
引用
收藏
页码：1179 / 1186
页数：8
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