Oxovanadium(IV) complexes of curcumin for cellular imaging and mitochondria targeted photocytotoxicity

被引：57
作者：
Banik, Bhabatosh [1 ]
Somyajit, Kumar [2 ]
Nagaraju, Ganesh [2 ]
Chakravarty, Akhil R. [1 ]
机构：
[1] Indian Inst Sci, Dept Inorgan & Phys Chem, Bangalore 560012, Karnataka, India
[2] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India
来源：
DALTON TRANSACTIONS | 2014年 / 43卷 / 35期
关键词：
DNA CLEAVAGE; ANTICANCER ACTIVITY; BINDING; CANCER; LIGHT; CYTOTOXICITY; DEGRADATION; IRRADIATION; DELIVERY; LIGANDS;
D O I：
10.1039/c4dt01487a
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
Oxovanadium(IV) complexes [VO(R-tpy)(cur)](ClO4) (1, 2) of curcumin (Hcur) and terpyridine ligands (R-tpy) where R is phenyl (phtpy in 1) or p-triphenylphosphonium methylphenyl bromide (C6H4CH2PPh3Br) (TPP-phtpy in 2) were prepared and characterized and their DNA photocleavage activity, photocytotoxicity and cellular localization in cancer cells (HeLa and MCF-7) were studied. Acetylacetonate (acac) complexes [VO(R-tpy)(acac)](ClO4) of phtpy (3) and TPP-phtpy (4) were prepared and used as the control species. These complexes showed efficient cleavage of pUC19 DNA in visible light of 454 nm and near-IR light of 705 rim. Complexes 1 and 2 showed significant photocytotoxicity in visible light of 400-700 nm. FACS analysis showed sub-G1/G0 phase cell-cycle arrest in cancer cells when treated with 1 and 2 in visible light in comparison with the dark controls. Fluorescence microscopic studies revealed specific localization of the p-triphenylphosphonium complex 2 in the mitochondria of MCF-7 cancer cells whereas no such specificity was observed for complex 1.
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页码：13358 / 13369
页数：12
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