Microbial nitrogen removal pathways in integrated vertical-flow constructed wetland systems

被引：116
作者：
Hu, Yun [1 ,2 ]
He, Feng [1 ]
Ma, Lin [1 ]
Zhang, Yi [1 ]
Wu, Zhenbin [1 ]
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 207卷
基金：
中国国家自然科学基金;
关键词：
Integrated vertical-flow constructed wetland; Nitrogen removal pathway; Isotope pairing technique; Nitrogen transformation functional gene; Anammox; ANAEROBIC AMMONIUM OXIDATION; HORIZONTAL SUBSURFACE-FLOW; DENITRIFYING BACTERIA; NOSZ GENES; ANAMMOX; NIRK; DENITRIFICATION; QUANTIFICATION; PERFORMANCE; COMMUNITY;
D O I：
10.1016/j.biortech.2016.01.106
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Microbial nitrogen (N) removal pathways in planted (Canna indica L.) and unplanted integrated vertical-flow constructed wetland systems (IVCWs) were investigated. Results of, molecular biological and isotope pairing experiments showed that nitrifying, anammox, and denitrifying bacteria were distributed in both down-flow and up-flow columns of the IVCWs. Further, the N transforming bacteria in the planted IVCWs were significantly higher than that in the unplanted ones (p < 0.05). Moreover, the potential nitrification, anammox, and denitrification rates were highest (18.90, 11.75, and 7.84 nmol N g (1) h (1), respectively) in the down-flow column of the planted IVCWs. Significant correlations between these potential rates and the absolute abundance of N transformation genes further confirmed the existence of simultaneous nitrification, anammox, and denitrification (SNAD) processes in the IVCWs. The anammox process was the major N removal pathway (55.6-60.0%) in the IVCWs. The results will further our understanding of the microbial N removal mechanisms in IVCWs. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：339 / 345
页数：7
相关论文
共 36 条
[1]   Shifts between Nitrospira- and Nitrobacter-like nitrite oxidizers underlie the response of soil potential nitrite oxidation to changes in tillage practices [J].
Attard, E. ;
Poly, F. ;
Commeaux, C. ;
Laurent, F. ;
Terada, A. ;
Smets, B. F. ;
Recous, S. ;
Le Roux, X. .
ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (02) :315-326
[2]   Treatment performance of integrated vertical-flow constructed wetland plots for domestic wastewater [J].
Chang, Jun-jun ;
Wu, Su-qing ;
Dai, Yan-ran ;
Liang, Wei ;
Wu, Zhen-bin .
ECOLOGICAL ENGINEERING, 2012, 44 :152-159
[3]   Effects of plant biomass on denitrifying genes in subsurface-flow constructed wetlands [J].
Chen, Yi ;
Wen, Yue ;
Zhou, Qi ;
Vymazal, Jan .
BIORESOURCE TECHNOLOGY, 2014, 157 :341-345
[4]   Microbial nitrogen transformation in constructed wetlands treating contaminated groundwater [J].
Coban, Oksana ;
Kuschk, Peter ;
Wells, Naomi S. ;
Strauch, Gerhard ;
Knoeller, Kay .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2015, 22 (17) :12829-12839
[5]   Nitrogen transforming community in a horizontal subsurface-flow constructed wetland [J].
Coban, Oksana ;
Kuschk, Peter ;
Kappelmeyer, Uwe ;
Spott, Oliver ;
Martienssen, Marion ;
Jetten, Mike S. M. ;
Knoeller, Kay .
WATER RESEARCH, 2015, 74 :203-212
[6]   Advanced nitrogen removal with simultaneous Anammox and denitrification in sequencing batch reactor [J].
Du, Rui ;
Peng, Yongzhen ;
Cao, Shenbin ;
Wu, Chengcheng ;
Weng, Dongchen ;
Wang, Shuying ;
He, Jianzhong .
BIORESOURCE TECHNOLOGY, 2014, 162 :316-322
[7]   The Contribution of Anammox and Denitrification to Sedirnent N2 Production in a Surface Flow Constructed Wetland [J].
Erler, Dirk V. ;
Eyre, Bradley D. ;
Davison, Leigh .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (24) :9144-9150
[8]   Microbial processes influencing performance of treatment wetlands: A review [J].
Faulwetter, Jennifer L. ;
Gagnon, Vincent ;
Sundberg, Carina ;
Chazarenc, Florent ;
Burr, Mark D. ;
Brisson, Jacques ;
Camper, Anne K. ;
Stein, Otto R. .
ECOLOGICAL ENGINEERING, 2009, 35 (06) :987-1004
[9]   Influence of macrophyte species on microbial density and activity in constructed wetlands [J].
Gagnon, V. ;
Chazarenc, F. ;
Comeau, Y. ;
Brisson, J. .
WATER SCIENCE AND TECHNOLOGY, 2007, 56 (03) :249-254
[10]   Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR [J].
Henry, S ;
Baudoin, E ;
López-Gutiérrez, JC ;
Martin-Laurent, F ;
Brauman, A ;
Philippot, L .
JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (03) :327-335
1234