Novel Pentapeptide Derived from Chicken by-Product Ameliorates DSS-Induced Colitis by Enhancing Intestinal Barrier Function via AhR-Induced Src Inactivation

被引：13

作者：

Li, Shanshan ^{[1
]}

Ma, Bin ^{[1
]}

Wang, Jin ^{[1
]}

Peng, Hengying ^{[1
]}

Zheng, Meng ^{[1
]}

Dai, Wenling ^{[1
]}

Liu, Jihua ^{[1
,2
]}

机构：

[1] China Pharmaceut Univ, Sch Tradit Chinese Pharm, Jiangsu Key Lab TCM Evaluat & Translat Res, Nanjing 211198, Peoples R China

[2] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 211198, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 48期

关键词：

Galli Gigeriae Endothelium Corneum; pentapeptide; colitis; intestinal barrier; aryl hydrocarbon receptor; Src; NF-kappa B; MAPK; ARYL-HYDROCARBON RECEPTOR; EPITHELIAL TIGHT JUNCTIONS; FAMILY KINASES; ACTIVATION; DYSFUNCTION; PERMEABILITY; DISRUPTION; STABILITY; INTEGRITY; OCCLUDIN;

D O I：

10.1021/acs.jafc.0c06319

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Managing patients with refractory inflammatory bowel diseases (IBD) is a common clinical challenge. Galli Gigeriae Endothelium Comeum (GGEC), a chicken by-product, has been used for centuries in Asian countries as a functional food and supplement for the treatment of gastrointestinal disorders. In this study, a novel peptide (LNLYP, LP-5) with gastrointestinal stability that can enhance the intestinal barrier function that was first identified in GGEC. Our work demonstrated that aryl hydrocarbon receptor (AhR) activation by LP-5 could inhibit the Src kinase to increase tight junction protein levels and down-regulate the expression of inflammatory cytokines to protect the intestinal barrier and finally alleviate dextran sulfate sodium (DSS)-induced colitis. This study revealed that LP-5 had the potential to develop into a therapeutic agent for the treatment of colitis and provided new high-valued utilization of GGEC.

引用

页码：14192 / 14203

页数：12

共 51 条

[1] Pathophysiology of IBD associated diarrhea [J].

Anbazhagan, Arivarasu N. ;

Priyamvada, Shubha ;

Alrefai, Waddah A. ;

Dudeja, Pradeep K. .

TISSUE BARRIERS, 2018, 6 (02)

[2] Molecular pathways controlling barrier function in IBD [J].

Atreya, Raja ;

Neurath, Markus F. .

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY, 2015, 12 (02) :67-68

[3] Tight Junction Proteins and Signaling Pathways in Cancer and Inflammation: A Functional Crosstalk [J].

Bhat, Ajaz A. ;

Uppada, Srijayaprakash ;

Achkar, Iman W. ;

Hashem, Sheema ;

Yadav, Santosh K. ;

Shanmugakonar, Muralitharan ;

Al-Naemi, Hamda A. ;

Haris, Mohammad ;

Uddin, Shahab .

FRONTIERS IN PHYSIOLOGY, 2019, 9

[4] The Aryl Hydrocarbon Receptor (AhR) Mediates the Counter-Regulatory Effects of Pelargonidins in Models of Inflammation and Metabolic Dysfunctions [J].

Biagioli, Michele ;

Carino, Adriana ;

Fiorucci, Chiara ;

Annunziato, Giannamaria ;

Marchiano, Silvia ;

Bordoni, Martina ;

Roselli, Rosalinda ;

Di Giorgio, Cristina ;

Castiglione, Federica ;

Ricci, Patrizia ;

Bruno, Agostino ;

Faccini, Andrea ;

Distrutti, Eleonora ;

Baldoni, Monia ;

Costantino, Gabriele ;

Fiorucci, Stefano .

NUTRIENTS, 2019, 11 (08)

[5] Intestinal barrier function in health and gastrointestinal disease [J].

Camilleri, M. ;

Madsen, K. ;

Spiller, R. ;

Van Meerveld, B. G. ;

Verne, G. N. .

NEUROGASTROENTEROLOGY AND MOTILITY, 2012, 24 (06) :503-512

[6] Effect of dehydroepiandrosterone on the immune response and gut microbiota in dextran sulfate sodium-induced colitis mice [J].

Cao, Ji ;

Zhang, Huihui ;

Yang, Zhongmiao ;

Zhao, Jinlong ;

Ma, Haitian .

MOLECULAR IMMUNOLOGY, 2020, 118 :60-72

[7] src family kinases regulate renal epithelial paracellular permeability barrier through an occludin-independent mechanism [J].

Caswell, Devin ;

Jaggi, Shuchie ;

Axis, Josephine ;

Amsler, Kurt .

JOURNAL OF CELLULAR PHYSIOLOGY, 2013, 228 (06) :1210-1220

[8] Intestinal Epithelial Barrier Dysfunction in Disease and Possible Therapeutical Interventions [J].

Catalioto, R. -M. ;

Maggi, C. A. ;

Giuliani, S. .

CURRENT MEDICINAL CHEMISTRY, 2011, 18 (03) :398-426

[9] Orally Administered CLA Ameliorates DSS-Induced Colitis in Mice via Intestinal Barrier Improvement, Oxidative Stress Reduction, and Inflammatory Cytokine and Gut Microbiota Modulation [J].

Chen, Yang ;

Yang, Bo ;

Ross, R. Paul ;

Jin, Yan ;

Stanton, Catherine ;

Zhao, Jianxin ;

Zhang, Hao ;

Chen, Wei .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (48) :13282-13298

[10] 2,3,7,8-Tetrachlorodibenzo-p-dioxin and up-regulation of neurofilament expression in neuronal cells: Evaluation of AhR and MAPK pathways [J].

Chen, Yangsheng ;

Xie, Heidi Qunhui ;

Sha, Rui ;

Xu, Tuan ;

Zhang, Songyan ;

Fu, Hualing ;

Xia, Yingjie ;

Liu, Yiyun ;

Xu, Li ;

Zhao, Bin .

ENVIRONMENT INTERNATIONAL, 2020, 134

← 1 2 3 4 5 6 →