Molecular markers of Ph1 gene in Chinese spring and development of winter wheat new line harboring ph1b gene

The genomic DNA of common wheat (Triticum aestivum L.) "Chinese Spring" (CS) and its ph1b mutant were analyzed by using 19 sequence tagged site PCR (STS-PCR) primers, which derived from RFLP probes from barley ( Hordeum vulgare L.) chromosome 5H. One marker was identified on wheat chromosome 5BL, which is 5.7 cM (centiMorgan) proximal to Ph1 gene, using the CS homoeologous group 5 nullisomic-tetrasomic, ditelosomic 5BL line and an F-2 population from CS x ph1b mutant. This linked PCR marker was converted into a more specific sequence characterized amplified region (SCAR) marker. To obtain a new winter wheat line containing phlb gene, the authors used a nullisomic 5B line of "Abbodanza" as a bridge parent and crossed respectively with the CS ph1b mutant (donor) and a winter wheat variety, "Jing 411" (recipient). The meiotic chromosome pairing was checked in the progeny of each cross, as well as using the marker-assistant selection of the SCAR marker identified for phlb gene.,After three inter-crossing and one selfing, a relatively stable ph1b substitution line of winter wheat with "Jing 411" background way obtained.