Preparation of anthraquinone high-yielding Senna alata extract and its stability

被引:9
作者
Sakunpak, A. [1 ]
Sirikatitham, A. [2 ]
Panichayupakaranant, R. [1 ]
机构
[1] Prince Songkla Univ, Fac Pharmaceut Sci, Dept Pharmacognosy & Pharmaceut Bot, Hat Yai 90112, Songkhla, Thailand
[2] Prince Songkla Univ, Fac Pharmaceut Sci, Dept Pharmaceut Chem, Hat Yai 90112, Songkhla, Thailand
关键词
Cassia alata; anthraquinone; emodin; aloe-emodin; antifungal;
D O I
10.1080/13880200802434757
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A one-step purification of Senna alata (L.) Roxb. (Leguminosae) extract using silica gel vacuum chromatographic technique provided an anthraquinone high-yielding S. alata leaf extract. This method was capable of improving its anthraquinone content as well as antifungal activity against dermatophytes. The extract contained anthraquinone content up to 16.7% w/w, and exhibited antifungal activity against Trichophyton rubrum, T mentagrophytes and Microsporum gypseum with minimum inhibitory concentration (MIC) values of 15.6, 62.5, and 250 mu g/mL, respectively. Stability evaluations of the anthraquinone high-yielding extract in several conditions through a period of 4 months found that the extracts were stable either kept under light or protected from light. The extracts were also stable under 4 +/- 2 degrees C, 30 +/- 2 degrees C and an accelerated condition, at 45 degrees C with 75% relative humidity. The aqueous solutions of the extracts, at pH 5.5 and 7, exhibited good stability. These results indicate that the anthraquinone high-yielding S. alata extract possessed satisfactory antifungal activity and stability for a further development of herbal medicines.
引用
收藏
页码:236 / 241
页数:6
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