Micronucleus Occurrence Related to Base Excision Repair Gene Polymorphisms in Chinese Workers Occupationally Exposed to Vinyl Chloride Monomer

Objective: To evaluate whether base excision repair gene polymorphisms contributed to susceptibility of chromosomal damage induced by vinyl, chloride monomer (VCM). Methods: Cytokinesis block micronucleus test. was per formed on 185 VCM-exposed workers to detect chromosomal damage in peripheral lymphocytes. The PCR-restriction fragment length polymorphism technique was applied to detect polymorphisms of TDG PARP1, and APE1 genes. Univariate and multivariate Poisson regression analysis was performed. Results: There was a 1.198-fold, increased micronucleus frequency,for individuals carrying TDG 199Gly/Ser + Ser/Ser genotypes compared with those carrying Gly/Gly genotype (P < 0.05). Individuals with the combined genotypes of TDG 199Gly/Ser + Ser/Ser and APE1 64Ile/Val + Val/Val had a significantly increased micronucleus, frequency compared, with those carrying wild-type genotypes for both genes. Conclusion: VCM-exposed workers carrying TDG 199Gly/Ser + Ser/Ser genotypes were susceptible to chromosomal damage. A combination of TDG Gly199Ser and, APE, Ile64Val, polymorphisms may modify the risk of chromosomal, damage. (J Occup Environ Med. 2009;51:578-585)