Interaction of Doxorubicin with Polynucleotides. A Spectroscopic Study

被引：70

作者：

Airoldi, Marta ^{[1
]}

Barone, Giampaolo ^{[1
]}

Gennaro, Giuseppe ^{[1
]}

Giuliani, Anna Maria ^{[1
]}

Giustini, Mauro ^{[2
]}

机构：

[1] Univ Palermo, Dipartimento STEBICEF, I-90128 Palermo, Italy

[2] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

BIOCHEMISTRY | 2014年 / 53卷 / 13期

关键词：

DNA-BINDING; DEOXYRIBONUCLEIC-ACID; ANTHRACYCLINE ANTIBIOTICS; CIRCULAR-DICHROISM; NUCLEIC-ACIDS; INTERCALATION; DAUNOMYCIN; COMPLEXES; DRUG; DAUNORUBICIN;

D O I：

10.1021/bi401687v

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of doxorubicin (DX) with model polynucleotides poly(dG-dC)center dot poly(dG-dC) (polyGC), poly(dA-dT)center dot poly(dA-dT) (polyAT), and calf thymus DNA has been studied by several spectroscopic techniques in phosphate buffer aqueous solutions. UV-vis, circular dichroism, and fluorescence spectroscopic data confirm that intercalation is the prevailing mode of interaction, and also reveal that the interaction with AT-rich regions leads to the transfer of excitation energy to DX not previously documented in the literature. Moreover, the DX affinity for AT sites has been found to be on the same order of magnitude as that reported for GC sites.

引用

页码：2197 / 2207

页数：11

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