The relationship of embryonic development, mortality, hatching success, and larval quality to normal or abnormal early embryonic cleavage in Atlantic cod, Gadus morhua

被引:42
作者
Avery, Trevor S. [1 ]
Killen, Shaun S. [2 ]
Hollinger, Tamara R. [3 ]
机构
[1] Acadia Univ, Dept Biol, Wolfville, NS B4P 2R6, Canada
[2] Univ Montpellier 2, Inst Sci Evolut Montpellier, Stn Mediterraneene Environm Littoral, F-34200 Sete, France
[3] Univ Prince Edward Isl, Atlantic Vet Coll, Charlottetown, PE C1A 4P3, Canada
关键词
Abnormal; Malformation; Blastomere; Egg quality; Asymmetric; LIFE-HISTORY TRAITS; BLASTOMERE MORPHOLOGY; EGG-PRODUCTION; FISH; VIABILITY;
D O I
10.1016/j.aquaculture.2008.12.011
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A reliable method for assessing the viability of fertilized eggs early in development would be beneficial for the aquaculture industry, allowing egg batches with a high probability of low hatching success to be discarded before costly resources are devoted to their culture, and for recruitment models where egg viability is used predictively. During the last decade, the observation of cellular morphology during embryogenesis has received increased attention as a potential early indicator of embryo quality. However, most often, abnormally cleaving eggs are assessed en masse even though noticeable differences in cleavage patterns are generally present between individual eggs. We separated six egg batches of Atlantic cod, Gadus morhua Linnaeus, 1758, into normal and abnormal cleavage patterns, reared eggs individually in a temperature-controlled room, and recorded daily egg mortality until hatch, hatching success, larval deformation, and larval mortality. Seven abnormal cleavage patterns were readily distinguishable and all showed moderate variability in egg mortality. Both normal and abnormal eggs had similar mortality-rate trends, consisting of an initial high mortality that became asymptotic at about day 8 of development at 6.5 degrees C. Specific cleavage patterns showed variable mortality-rate trends. No significant differences in cumulative egg mortality were found between any abnormal cleavage patterns, but overall, abnormal eggs had significantly greater egg mortality than normal eggs. Hatching success was high in all groups and not significantly different between normal and abnormal eggs. Few larvae were deformed within any egg batch or pattern and no consistent trends were noted. A severity index was calculated and a suggested severity order determined as asymmetry <adhesions< margins <= inclusions< blastomere size. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:265 / 273
页数:9
相关论文
共 35 条
[1]   Investigating the relationship among abnormal patterns of cell cleavage, egg mortality and early larval condition in Limanda ferruginea [J].
Avery, TS ;
Brown, JA .
JOURNAL OF FISH BIOLOGY, 2005, 67 (03) :890-896
[2]  
AVERY TS, 2001, THESIS MEMORIAL U NE
[3]  
Bromage N.R., 1995, Broodstock Management and Egg and Larval Quality, P1
[4]  
Bromage Niall, 1994, Journal of the World Aquaculture Society, V25, P13, DOI 10.1111/j.1749-7345.1994.tb00799.x
[5]   Quality in fish: what makes a good egg? [J].
Brooks, S ;
Tyler, CR ;
Sumpter, JP .
REVIEWS IN FISH BIOLOGY AND FISHERIES, 1997, 7 (04) :387-416
[6]   MORPHOLOGICAL AND CHROMOSOMAL-ABERRATIONS DURING EMBRYONIC-DEVELOPMENT IN DAB LIMANDA-LIMANDA [J].
CAMERON, P ;
BERG, J .
MARINE ECOLOGY PROGRESS SERIES, 1992, 91 (1-3) :163-169
[7]   Biological effects monitoring of the North Sea employing fish embryological data [J].
Cameron, P ;
Berg, J ;
VonWesternhagen, H .
ENVIRONMENTAL MONITORING AND ASSESSMENT, 1996, 40 (02) :107-124
[8]  
CHAMBERS RC, 1989, FISH B-NOAA, V87, P515
[9]   Stages of embryonic development in the Atlantic cod Gadus morhua [J].
Hall, TE ;
Smith, P ;
Johnston, IA .
JOURNAL OF MORPHOLOGY, 2004, 259 (03) :255-270
[10]  
Kane D.A., 2004, P157