The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei

被引：16
作者：
Zikova, Alena
Horakova, Eva
Jirku, Milan
Dunajcikova, Petra
Lukes, Julius
机构：
[1] Acad Sci Czech Republic, Inst Parasitol, CR-37005 Ceske Budejovice, Czech Republic
[2] Univ S Bohemia, Fac Biol, CR-37005 Ceske Budejovice, Czech Republic
[3] Comenius Univ, Fac Nat Sci, Bratislava, Slovakia
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2006年 / 149卷 / 01期
关键词：
mitochondrial respiratory chain; alternative oxidase; trypanosome; RNA interference; RNA editing; RNA-binding proteins;
D O I：
10.1016/j.molbiopara.2006.04.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MRP1 and MRP2 are multifunctional mitochondrial RNA-binding proteins with a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Silencing of MRP1 and/or MRP2 by RNA interference affected the assembly and functionality of respiratory complexes. The absence of several subunits of complexes I, III and IV resulted in their disintegration and subsequent decrease of specific activities and also caused a significant decrease of membrane potential. The overall respiration in the interfered cells decreased by only about 20%, since the trypanosome alternative oxidase effectively replaced the missing cytochromes and became the principal terminal oxidase. (c) 2006 Elsevier B.V. All rights reserved.
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收藏
页码：65 / 73
页数：9
相关论文
共 37 条
[1]   A 100-kD complex of two RNA-binding proteins from mitochondria of Leishmania tarentolae catalyzes RNA annealing and interacts with several RNA editing components [J].
Aphasizhev, R ;
Aphasizheva, I ;
Nelson, RE ;
Simpson, L .
RNA, 2003, 9 (01) :62-76
[2]   Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase):: The key enzyme in U-insertion/deletion RNA editing [J].
Aphasizhev, R ;
Sbicego, S ;
Peris, M ;
Jang, SH ;
Aphasizheva, I ;
Simpson, AM ;
Rivlin, A ;
Simpson, L .
CELL, 2002, 108 (05) :637-648
[3]  
ATTARDI G, 1988, ANNU REV CELL BIOL, V4, P289, DOI 10.1146/annurev.cb.04.110188.001445
[4]   The presence of rotenone-sensitive NADH dehydrogenase in the long slender bloodstream and the procyclic forms of Trypanosoma brucei brucei [J].
Beattie, DS ;
Howton, MM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (03) :888-894
[5]   Energy generation in insect stages of Trypanosoma brucei:: metabolism in flux [J].
Besteiro, S ;
Barrett, MP ;
Rivière, L ;
Bringaud, F .
TRENDS IN PARASITOLOGY, 2005, 21 (04) :185-191
[6]   Cloning and characterization of two guide RNA-binding proteins from mitochondria of Crithidia fasciculata:: gBP27, a novel protein, and gBP29, the orthologue of Trypanosoma brucei gBP21 [J].
Blom, D ;
van den Burg, J ;
Breek, CKD ;
Speijer, D ;
Muijsers, AO ;
Benne, R .
NUCLEIC ACIDS RESEARCH, 2001, 29 (14) :2950-2962
[7]   ATP synthase is responsible for maintaining mitochondrial membrane potential in bloodstream form Trypanosoma brucei [J].
Brown, SV ;
Hosking, P ;
Li, JL ;
Williams, N .
EUKARYOTIC CELL, 2006, 5 (01) :45-53
[8]   ATP generation in the Trypanosoma brucei procyclic form -: Cytosolic substrate level phosphorylation is essential, but not oxidative phosphorylation [J].
Coustou, V ;
Besteiro, S ;
Biran, M ;
Diolez, P ;
Bouchaud, V ;
Voisin, P ;
Michels, PAM ;
Canioni, P ;
Baltz, T ;
Bringaud, F .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (49) :49625-49635
[9]   Uridine insertion/deletion RNA editing in trypanosome mitochondria -: a review [J].
Estévez, AM ;
Simpson, L .
GENE, 1999, 240 (02) :247-260
[10]   NADH-ubiquinone oxidoreductase activity in the kinetoplasts of the plant trypanosornatid Phytomonas serpens [J].
González-Halphen, D ;
Maslov, DA .
PARASITOLOGY RESEARCH, 2004, 92 (04) :341-346
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