Isomaltose formation by free and immobilized dextransucrase

被引：11
作者：
Berensmeier, Sonja
Joerdening, Hans-Joachim
Buchholz, Klaus
机构：
[1] Tech Univ Carolo Wilhelmina Braunschweig, Dept Carbohydrates, D-38106 Braunschweig, Germany
[2] Forschungszentrum Karlsruhe, Inst Tech Chem, D-76021 Karlsruhe, Germany
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 04期
关键词：
alginate; dextransucrase; fluidized bed; immobilization; isomaltooligosaccharide;
D O I：
10.1080/10242420500491854
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.
引用
收藏
页码：280 / 290
页数：11
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