Evaluation of a Short, On-Plate Formic Acid Extraction Method for Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Identification of Clinically Relevant Yeast Isolates

被引：17

作者：

Gorton, Rebecca L. ^{[1
,2
]}

Seaton, Shila ^{[3
]}

Ramnarain, Purnima ^{[1
]}

McHugh, Timothy D. ^{[2
]}

Kibbler, Christopher C. ^{[1
,2
]}

机构：

[1] Royal Free Hosp, Dept Med Microbiol, UK Clin Mycol Network UKCMN Reg Lab, London NW3 2QG, England

[2] UCL, Ctr Clin Microbiol, London, England

[3] UK NEQAS Publ Hlth England, London, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 04期

关键词：

MALDI-TOF-MS; BRUKER BIOTYPER; MYCOLOGY; FUNGI;

D O I：

10.1128/JCM.03489-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This report describes a short, on-plate formic acid (FA) extraction method for the identification of clinical yeast isolates using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A total of 41.1% (78/190) and 63.7% (121/190) of yeasts were identified using species log score thresholds of > 2.0 and > 1.9, respectively. Overall, 97.4% (185/190) of yeasts were identified in combination with conventional FA extraction.

引用

页码：1253 / 1255

页数：3

共 15 条

[1] Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [J].

Bader, O. ;

Weig, M. ;

Taverne-Ghadwal, L. ;

Lugert, R. ;

Gross, U. ;

Kuhns, M. .

CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (09) :1359-1365

[2] MALDI-TOF-MS-based species identification and typing approaches in medical mycology [J].

Bader, Oliver .

PROTEOMICS, 2013, 13 (05) :788-799

[3] Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identification in the routine clinical laboratory [J].

Cassagne, Carole ;

Cella, Anne-Laure ;

Suchon, Pierre ;

Normand, Anne-Cecile ;

Ranque, Stephane ;

Piarroux, Renaud .

MEDICAL MYCOLOGY, 2013, 51 (04) :371-377

[4] Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Routine Identification of Yeast [J].

Dhiman, Neelam ;

Hall, Leslie ;

Wohlfiel, Sherri L. ;

Buckwalter, Seanne P. ;

Wengenack, Nancy L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1614-1616

[5] Routine Identification of Medical Fungi by the New Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight System with a New Time-Effective Strategy [J].

Iriart, Xavier ;

Lavergne, Rose-Anne ;

Fillaux, Judith ;

Valentin, Alexis ;

Magnaval, Jean-Francois ;

Berry, Antoine ;

Cassaing, Sophie .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (06) :2107-2110

[6] Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practice [J].

Jenkins, Claire ;

Ling, Clare L. ;

Ciesielczuk, Holly L. ;

Lockwood, Julianne ;

Hopkins, Susan ;

McHugh, Timothy D. ;

Gillespie, Stephen H. ;

Kibbler, Christopher C. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 61 (04) :483-488

[7] Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance [J].

Mancini, Nicasio ;

De Carolis, Elena ;

Infurnari, Laura ;

Vella, Antonietta ;

Clementi, Nicola ;

Vaccaro, Luisa ;

Ruggeri, Alberto ;

Posteraro, Brunella ;

Burioni, Roberto ;

Clementi, Massimo ;

Sanguinetti, Maurizio .

JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (07) :2453-2457

[8] Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates [J].

Marklein, G. ;

Josten, M. ;

Klanke, U. ;

Mueller, E. ;

Horre, R. ;

Maier, T. ;

Wenzel, T. ;

Kostrzewa, M. ;

Bierbaum, G. ;

Hoerauf, A. ;

Sahl, H. -G. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (09) :2912-2917

[9]

Posterard B, 2013, EXPERT REV PROTEOMIC, V10, P151, DOI [10.1586/EPR.13.8, 10.1586/epr.13.8]

[10] Performance of matrix-assisted laser desorption-time of flight mass spectrometry for identification of clinical yeast isolates [J].

Rosenvinge, Flemming S. ;

Dzajic, Esad ;

Knudsen, Elisa ;

Malig, Sanne ;

Andersen, Line B. ;

Lovig, Annette ;

Arendrup, Maiken C. ;

Jensen, Thoger G. ;

Gahrn-Hansen, Bente ;

Kemp, Michael .

MYCOSES, 2013, 56 (03) :229-235

← 1 2 →