An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

被引:24
作者
Huang, Juan [1 ,2 ]
Jia, Renyong [1 ,2 ,3 ]
Wang, Mingshu [1 ,2 ,3 ]
Shu, Bing [1 ,2 ]
Yu, Xia [1 ,2 ]
Zhu, Dekang [2 ,3 ]
Chen, Shun [1 ,2 ,3 ]
Yin, Zhongqiong [3 ]
Chen, Xiaoyue [2 ,3 ]
Cheng, Anchun [1 ,2 ,3 ]
机构
[1] Sichuan Agr Univ, Inst Prevent Vet Med, Chengdu, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Coll Vet Med, Avian Dis Res Ctr, Yaan, Sichuan, Peoples R China
[3] Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu, Sichuan, Peoples R China
关键词
T-CELL RESPONSES; INFLUENZA-A VIRUS; ENTERITIS VIRUS; MEDIATED PROTECTION; HERPESVIRUS; DUCKLINGS; IMMUNIZATION; MICE; IGA; REPLICATION;
D O I
10.1128/CVI.00605-13
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Duck plague (DP) is a severe disease caused by DP virus (DPV). Control of the disease is recognized as one of the biggest challenges in avian medicine. Vaccination is an efficient way to control DPV, and an attenuated vaccine is the main routine vaccine. The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic and mucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after subcutaneous immunization of ducks. CD4(+) and CD8(+) T cells were counted by flow cytometry, and humoral and mucosal Ig anti-bodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig antibodies were present postimmunization and that there were more CD4(+) T cells than CD8(+) T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected ducks against 1,000 50% lethal doses (LD50) of the lethal DPV strain CHv via oral infection. These data suggest that this subcutaneous vaccine elicits sufficient systemic and mucosal immune responses against lethal DPV challenge to be protective in ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in ducks.
引用
收藏
页码:457 / 462
页数:6
相关论文
共 43 条
[31]   Replication kinetics of duck virus enteritis vaccine virus in ducklings immunized by the mucosal or systemic route using real-time quantitative PCR [J].
Qi, Xuefeng ;
Yang, Xiaoyan ;
Cheng, Anchun ;
Wang, Mingshu ;
Guo, Yufei ;
Jia, Renyong .
RESEARCH IN VETERINARY SCIENCE, 2009, 86 (01) :63-67
[32]   Induction of Homologous Rather than Heterologous Antigen-Specific CD4 T Cell Responses Is Critical for Functional CD8 T Cell Responses in Mice Transgenic for a Foreign Antigen [J].
Sabarth, Nicolas ;
Chamberlain, Louise ;
Brett, Sara ;
Tite, John ;
Craigen, Jenny .
JOURNAL OF IMMUNOLOGY, 2010, 185 (08) :4590-4601
[33]   Immune response to intranasal and intraperitoneal immunization with Kaposi's sarcoma-associated herpesvirus in mice [J].
Sakamoto, Kouta ;
Asanuma, Hideki ;
Nakamura, Tomoyuki ;
Kanno, Takayuki ;
Sata, Tetsutaro ;
Katano, Harutaka .
VACCINE, 2010, 28 (19) :3325-3332
[34]   Inactivated vaccine for protection against duck virus enteritis [J].
Shawky, SA ;
Sandhu, TS .
AVIAN DISEASES, 1997, 41 (02) :461-468
[35]   Development and application of an indirect immunohistochemical method for the detection of duck plague virus vaccine antigens in paraffin sections and localization in the vaccinated duckling tissues [J].
Shen, F. X. ;
Ma, G. P. ;
Cheng, A. C. ;
Wang, M. S. ;
Li, C. F. ;
Sun, K. F. ;
Chang, H. ;
Zhu, D. K. ;
Jia, R. Y. ;
Chen, X. Y. ;
Sun, T. .
POULTRY SCIENCE, 2010, 89 (09) :1915-1923
[36]   Virulence of six strains of duck plague virus in eight waterfowl species [J].
Spieker, JO ;
Yuill, TM ;
Burgess, EC .
JOURNAL OF WILDLIFE DISEASES, 1996, 32 (03) :453-460
[37]   Focus issue: Structure and function of lymphoid tissues [J].
Thompson, Elizabeth C. .
TRENDS IN IMMUNOLOGY, 2012, 33 (06) :255-255
[38]   Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine [J].
Tokuhara, Daisuke ;
Yuki, Yoshikazu ;
Nochi, Tomonori ;
Kodama, Toshio ;
Mejima, Mio ;
Kurokawa, Shiho ;
Takahashi, Yuko ;
Nanno, Masanobu ;
Nakanishi, Ushio ;
Takaiwa, Fumio ;
Honda, Takeshi ;
Kiyono, Hiroshi .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (19) :8794-8799
[39]  
TOTH TE, 1970, AM J VET RES, V31, P1275
[40]   Lymphotoxin Beta Receptor Signaling in Intestinal Epithelial Cells Orchestrates Innate Immune Responses against Mucosal Bacterial Infection [J].
Wang, Yugang ;
Koroleva, Ekaterina P. ;
Kruglov, Andrei A. ;
Kuprash, Dmitry V. ;
Nedospasov, Sergei A. ;
Fu, Yang-Xin ;
Tumanov, Alexei V. .
IMMUNITY, 2010, 32 (03) :403-413