Liver sinusoidal endothelial cells (LSECs) modifications in patients with chronic hepatitis C

被引：41

作者：

Baiocchini, Andrea ^{[1
]}

Del Nonno, Franca ^{[1
]}

Taibi, Chiara ^{[2
]}

Visco-Comandini, Ubaldo ^{[2
]}

D'Offizi, Gianpiero ^{[2
]}

Piacentini, Mauro ^{[3
,4
]}

Falasca, Laura ^{[4
]}

机构：

[1] Natl Inst Infect Dis Lazzaro Spallanzani IRCCS, Dept Epidemiol & Preclin Res, Pathol Unit, Rome, Italy

[2] Natl Inst Infect Dis Lazzaro Spallanzani IRCCS, POIT Dept, Infect Dis Hepatol Unit, Rome, Italy

[3] Univ Roma Tor Vergata, Dept Biol, Rome, Italy

[4] Natl Inst Infect Dis Lazzaro Spallanzani IRCCS, Dept Epidemiol & Preclin Res, Lab Elect Microscopy, Rome, Italy

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

FIBROSIS; INJURY; CAPILLARIZATION; ANGIOGENESIS; EXPRESSION; CAVEOLIN-1; DISEASE;

D O I：

10.1038/s41598-019-45114-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The sinusoidal endothelial cells present in the liver (LSECs) are tipically characterized by the presence of pores (fenestrae). During some pathological conditions LSECs undergo "capillarization", a process characterized by loss of fenestrations and acquisition of a vascular phenotype. In chronic liver disease capillarization has been reported to precede the development of fibrosis. LSECs modification in the setting of HCV infection is currently poorly investigated. Considering that HCV accounts for important changes in hepatocytes and in view of the intimate connection between hepatocytes and LSECs, here we set out to study in great detail the LSECs modifications in individuals with HCV-dependent chronic hepatitis. Electron microscopy analysis, and evaluation of CD32, CD31 and caveolin-1 expression showed that in HCV infection LSECs display major morphological changes but maintain their phenotypical identity. Capillarization was observed only in cases at initial stages of fibrosis. Our findings showed that the severity of LSECs modifications appears to be correlated with hepatocytes damage and fibrosis stage providing novel insight in the pathogenesis of HCV-chronic hepatitis.

引用

页数：10

共 44 条

[11] Hepatic sinusoids in liver injury, inflammation, and fibrosis: new pathophysiological insights [J].