Assessing sirtuin expression in endometrial carcinoma and non-neoplastic endometrium

被引:76
作者
Bartosch, Carla [1 ,2 ,3 ]
Monteiro-Reis, Sara [1 ,2 ]
Almeida-Rios, Diogo [1 ,2 ]
Vieira, Renata [1 ]
Castro, Armando [4 ]
Moutinho, Manuel [4 ]
Rodrigues, Marta [4 ]
Graca, Ines [2 ]
Lopes, Jose Manuel [3 ,4 ,5 ]
Jeronimo, Carmen [2 ,6 ]
机构
[1] Portuguese Oncol Inst Porto IPO Porto, Dept Pathol, Oporto, Portugal
[2] Portuguese Oncol Inst Porto, Res Ctr, Canc Epigenet & Biol Grp, Oporto, Portugal
[3] Univ Porto, Fac Med, Dept Pathol & Oncol, Rua Campo Alegre 823, P-4100 Oporto, Portugal
[4] CHSJ, Dept Pathol, Oporto, Portugal
[5] Univ Porto, IPATIMUP Inst Mol Pathol & Immunol, Rua Campo Alegre 823, P-4100 Oporto, Portugal
[6] Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Dept Pathol & Mol Immunol, Rua Campo Alegre 823, P-4100 Oporto, Portugal
关键词
endometrial carcinoma; endometrium; sirtuin; SIRT1; SIRT7; Pathology Section; CELL-PROLIFERATION; BREAST-CANCER; PROMOTES; RECEPTOR; P53; IMMUNOHISTOCHEMISTRY; METABOLISM; STRESS; GROWTH;
D O I
10.18632/oncotarget.6691
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Sirtuins participate in hormone imbalance, metabolism and aging, which are important processes for endometrial cancer (EC) development. Sirtuins mRNA expression (SIRT1 to 7) was determined in 76 ECs (63 Type I, 12 Type II and one mixed EC), and 30 non-neoplastic endometria (NNE) by quantitative real-time PCR. SIRT1 and SIRT7 protein expression was evaluated by immunohistochemistry using Allred score. Compared to NNE, ECs showed SIRT7 (p < 0.001) mRNA overexpression, whereas SIRT1 (p < 0.001), SIRT2 (p < 0.001), SIRT4 (p < 0.001) and SIRT5 (p < 0.001) were underexpressed. No significant differences were observed for SIRT3 and SIRT6. Type II ECs displayed lower SIRT1 (p = 0.032) and SIRT3 (p = 0.016) transcript levels than Type I ECs. Concerning protein expression, SIRT1 immunostaining median score was higher in ECs compared to NNE epithelium (EC = 5 vs. NNE = 2, p < 0.001), while SIRT7 was lower in ECs (EC = 6 vs. NNE = 7, p < 0.001). No significant associations were found between SIRT1/7 immunoexpression and histological subtype, grade, lymphovascular invasion or stage. Our data shows that sirtuins are deregulated in EC. The diversity of expression patterns observed suggests that sirtuins may have distinctive roles in endometrial cancer similarly to what has been described in other cancer models.
引用
收藏
页码:1144 / 1154
页数:11
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