Carbonic anhydrase inhibitors. Inhibition of the fungal β-carbonic anhydrases from Candida albicans and Cryptococcus neoformans with boronic acids

Inhibition of the beta-carbonic anhydrases (CAs, EC 4.2.1.1) from the pathogenic fungi Cryptococcus neoformans (Can2) and Candida albicans (Nce103) with a series of aromatic, arylalkenyl- and arylalkylboronic acids was investigated. Aromatic, 4-phenylsubstituted- and 2-naphthylboronic acids were the best Can2 inhibitors, with inhibition constants in the range of 8.5-11.5 mu M, whereas arylalkenyl and aryalkylboronic acids showed K(I)s in the range of 428-3040 mu M. Nce103 showed a similar inhibition pro. le, with the 4-phenylsubstituted- and 2-naphthylboronic acids possessing K(I)s in the range of 7.8-42.3 mu M, whereas the arylalkenyl and aryalkylboronic acids were weaker inhibitors (K(I)s of 412-5210 mu M). The host human enzymes CAI and II were also effectively inhibited by these boronic acids. The B(OH)(2) moiety is thus a new zinc-binding group for designing effective inhibitors of the alpha- and beta-CAs. (C) 2009 Elsevier Ltd. All rights reserved.