Paraoxonase-1 inhibits oxidised LDL-induced MCP-1 production by endothelial cells

被引:162
作者
Mackness, B [1 ]
Hine, D [1 ]
Liu, YF [1 ]
Mastorikou, M [1 ]
Mackness, M [1 ]
机构
[1] Manchester Royal Infirm, Dept Med, Manchester M13 9WL, Lancs, England
关键词
paraoxonase-1; HDL; MCP-1; oxidation; endothelial cells;
D O I
10.1016/j.bbrc.2004.04.056
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The upregulation of endothelial cell MCP-1 production by ox-LDL is a major initiating event in atherogenesis. HDL and PON1 retard the oxidation of LDL and therefore may retard endothelial cell MCP-1 production. The endothelial cell line EAhy926 was incubated with ox-LDL in the presence and absence of HDL and PON1 and the production of MCP-1 was measured by ELISA. Human HDL and PON1 significantly inhibited the in vitro oxidation of LDL and completely prevented the ox-LDL induced increase in MCP-1 production by endothelial cells. Ostrich HDL that does not contain PON1 was unable to prevent LDL-oxidation or the production of MCP-1 by endothelial cells. PON1 attenuates the ox-LDL induced MCP-1 production by endothelial cells. This is one, early, mechanism by which PON1 may be anti-atherogenic. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:680 / 683
页数:4
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