The Therapeutic Effect of Extracellular Superoxide Dismutase (EC-SOD) Mouse Embryonic Fibroblast (MEF) on Collagen-Induced Arthritis (CIA) Mice

被引:10
作者
Yu, Dong Hoon
Kim, Myoung Ok
Kim, Sung Hyun
Shin, Mi Jung
Kim, Bong Soo
Kim, Hei Jung
Lee, Sang Ryeul [3 ]
Lee, Sang Gyu
Yoo, Seung-Ah [5 ]
Kim, Wan Uk [5 ]
Hyun, Byung Hwa [4 ]
Park, Young Sik [2 ]
Kim, Tae Yoon [3 ]
Ryoo, Zae Young [1 ]
机构
[1] Kyungpook Natl Univ, Sch Life Sci & Biotechnol, Coll Nat Sci, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Sch Life & Food Sci, Taegu 702701, South Korea
[3] Catholic Univ, Coll Med, Dept Immunol & Dermatol, Seoul 137040, South Korea
[4] Korea Res Inst Biosci & Biotechnol, Dis Model Res Ctr, Taejon 305806, South Korea
[5] Catholic Univ, Dept Internal Med, Div Rheumatol, Seoul, South Korea
关键词
Rheumatoid arthritis; Collagen-induced arthritis (CIA); Extracellular superoxide dismutase (EC-SOD); Mouse embryonic fibroblast (MEF);
D O I
10.3727/096368908787648029
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Rheumatoid arthritis is a chronic inflammatory disease. The generation of reactive oxygen species (ROS) within an inflamed joint has been suggested as playing a significant pathogenic role. Extracellular Superoxide dismutase (EC-SOD) is a major scavenger enzyme of ROS, which has received growing attention for its therapeutic potential. To investigate the therapeutic effect of EC-SOD in mice with collagen-induced arthritis (CIA), we used mouse embryonic Fibroblast (MEF) of transgenic mice that overexpresses EC-SOD on the skin by using hK14 promoter. DBA/1 mice that had been treated with bovine type II collagen were administrated subcutaneous injections of EC-SOD transgenic MEF (each at 1.4 x 10(6) Cells) on days 28, 35, and 42 after primary immunization. To test EC-SOD activity, blood samples were collected in each group on day 49. The EC-SOD activity was nearly 1.5-fold higher in the transgenic MEF-treated group than in the nontransgenic MEF-treated group (p < 0.05). The severity of arthritis in mice was scored in a double-blind manner. with each paw being assigned a separate clinical score. The severity of arthritis in EC-SOD transgenic MEF-treated mice was significantly Suppressed in the arthritic clinical score (p < 0.05). To investigate the alteration of cytokine levels, ELISA was used to measure blood samples. Levels of IL-I beta and TNF-alpha were reduced in the transgenic MEF-treated group (p < 0.05). Abnormalities of the joints were examined by H&E staining. There were no signs of inflammation except for mild hyperplasia of the synovium in the transgenic MEF-treated group. ne proliferation of CII-specific T cells was lower in the transgenic MEF-treated mice than in those in the other groups. The transfer of EC-SOD transgenic MEF has shown a therapeutic effect in CIA mice and this approach may be a safer and more effective form of therapy for rheumatoid arthritis.
引用
收藏
页码:1371 / 1380
页数:10
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