Newcastle disease virus surveillance in Argentina: Use of reverse transcription-polymerase chain reaction and sequencing for molecular typification

Newcastle disease virus (NDV) remains a major pathogen of poultry where highly virulent strains require reporting to the Office of International Epizootes. NDV is a paramyxovirus existing as different strains classified on the basis of severity of the disease they cause. The present study was conducted in Argentina to determine the prevalence of highly virulent velogenic NDV strains in commercial poultry farms. Tracheal and cloacal swabs from 693 flocks, representing 14% of the broiler production, were collected and pooled. A pool amplified twice in embryonated eggs presented a limited hemagglutination titer. We performed reverse transcription coupled to polymerase chain reaction to amplify fusion and matrix protein gene sequences of the isolate and the strain Trenque Lauquen, isolated in Argentina during an outbreak in 1970-71 and previously characterized as velogenic viscerotropic by biological methods. The amino acid sequences were deduced From nucleotide sequences of the amplification products and the pathotype predicted according to the sequences obtained. From the samples analyzed, we found only one type of NDV, being the isolate identified as lentogenic NDV. This strain is probably the one used in vaccination of flocks where that sample was obtained. These data have allowed us to consider a velogenic NDV-free status in Argentina's commercia poultry