Determination of ochratoxin A in coriander (Coriandrum sativum L.) by hpcl/fluorescence detection

An analytical study based on extraction with acetonitrile-water, immunoaffinity column cleanup, and HPLC/fluorescence detection for separation and identification of ochratoxin A in coriander was carried out. Validation of the applied methodology was done through accuracy and precision studies. Homogenized samples of coriander were spiked in triplicate with ochratoxin A at 0.5, 1.0, 2.0, and 5.0 mu g/kg levels. Recovery values were in the range of 98% for a fortification level at 0.5 mu g/kg to 109.1% at 1.0 mu g/kg. Application to coriander samples available in Portuguese markes showed no contamination with ochratoxin A.