Determination of two C21 steroidal glycosides of Baishouwu and their metabolites in mice plasma and tumor homogenate by liquid chromatography-tandem mass spectrometry

被引：12

作者：

Xu, Wen ^{[1
]}

Shan, Lei ^{[1
]}

Liu, Xiaohua ^{[1
]}

Li, Haiyun ^{[1
]}

Hu, Liwei ^{[1
]}

Zhang, Weidong ^{[1
,2
]}

机构：

[1] Second Mil Med Univ, Sch Pharm, Dept Nat Med Chem, Shanghai 200433, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 876卷 / 02期

关键词：

Liquid chromatography-tandem mass spectrometry; Wilfoside C3N; Wilfoside C1N; Metabolites; Mice plasma; Tumor homogenate; Deoxy sugar;

D O I：

10.1016/j.jchromb.2008.10.049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For the evaluating of the absorption, tumor affinity and metabolism of wilfoside C3N and wilfoside C1N (two main C21 steroidal glycosides from Baishouwu), an LC-MS/MS method was developed. Plasma or tumor homogenate samples were extracted by liquid-liquid extraction with ethyl acetate after internal standard (ginsenoside Rh2) spiked. The separation was performed by a Luna C18 column (3.0 mu m, 2.0 mm x 50 mm) with gradient elution. The method was fully validated and successfully applied to determine the concentrations of the parent drugs and metabolites after intragastric administration of wilfoside C3N and wilfoside C1N to mice respectively. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：203 / 210

页数：8

共 16 条

[1] The type of sugar moiety is a major determinant of the small intestinal uptake and subsequent biliary excretion of dietary quercetin glycosides [J].

Arts, ICW ;

Sesink, ALA ;

Faassen-Peters, M ;

Hollman, PCH .

BRITISH JOURNAL OF NUTRITION, 2004, 91 (06) :841-847

[2] Intestinal sugar transport [J].

Drozdowski, Laurie A. ;

Thomson, Alan B. R. .

WORLD JOURNAL OF GASTROENTEROLOGY, 2006, 12 (11) :1657-1670

[3]

Guo Z Z, 1988, Zhong Xi Yi Jie He Za Zhi, V8, P282

[4] Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings (Reprinted from Advanced Drug Delivery Reviews, vol 23, pg 3-25, 1997) [J].

Lipinski, CA ;

Lombardo, F ;

Dominy, BW ;

Feeney, PJ .

ADVANCED DRUG DELIVERY REVIEWS, 2001, 46 (1-3) :3-26

[5] Factors that cause the β-anomeric preference of Na+/glucose cotransporter for intestinal transport of monosaccharide conjugates [J].

Mizuma, T ;

Nagamine, Y ;

Dobashi, A ;

Awazu, S .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1998, 1381 (03) :340-346

[6] THE BETA-ANOMERIC AND GLUCOSE PREFERENCES OF GLUCOSE-TRANSPORT CARRIER FOR INTESTINAL ACTIVE ABSORPTION OF MONOSACCHARIDE CONJUGATES [J].

MIZUMA, T ;

OHTA, K ;

AWAZU, S .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1994, 1200 (02) :117-122

[7]

PENG YR, 2008, PHYTOMEDICINE

[8] Determination of caudatin-2,6-dideoxy-3-O-methy-β-D-cymaropyranoside in rat plasma using liquid chromatography-tandem mass spectrometry [J].

Peng, Yunru ;

Li, Youbin ;

Wang, Dawei ;

Liu, Xiaodong ;

Zhang, Jianfeng ;

Qian, Shihui ;

Duan, Jinao .

BIOMEDICAL CHROMATOGRAPHY, 2008, 22 (06) :575-580

[9] Substrate specificity of sugar transport by rabbit SGLT1: Single-molecule atomic force microscopy versus transport studies [J].

Puntheeranurak, Theeraporn ;

Wimmer, Barbara ;

Castaneda, Francisco ;

Gruber, Hermann J. ;

Hinterdorfer, Peter ;

Kinne, Rolf K. H. .

BIOCHEMISTRY, 2007, 46 (10) :2797-2804

[10] Breast cancer resistance protein (Bcrp1/Abcg2) limits net intestinal uptake of quercetin in rats by facilitating apical efflux of Glucuronides [J].

Sesink, ALA ;

Arts, ICW ;

de Boer, VCJ ;

Breedveld, P ;

Schellens, JHM ;

Hollman, PCH ;

Russel, FGM .

MOLECULAR PHARMACOLOGY, 2005, 67 (06) :1999-2006

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