Mechanism of Interleukin-4 Reducing Lipid Deposit by Regulating Hormone-Sensitive Lipase

被引：37

作者：

Shiau, Ming-Yuh ^{[1
]}

Chuang, Pei-Hua ^{[2
]}

Yang, Ching-Ping ^{[2
]}

Hsiao, Chiao-Wan ^{[2
,3
,4
]}

Chang, Shu-Wen ^{[2
]}

Chang, Kai-Yun ^{[2
]}

Liu, Tsung-Ming ^{[2
]}

Chen, Huan-Wen ^{[2
]}

Chuang, Cheng-Chieh ^{[2
]}

Yuan, Sheau-Yun ^{[1
,5
]}

Chang, Yih-Hsin ^{[2
]}

机构：

[1] Hungkuang Univ, Dept Nursing, Coll Nursing, Taichung, Taiwan

[2] Natl Yang Ming Univ, Dept Biotechnol & Lab Sci Med, Taipei, Taiwan

[3] Natl Yang Ming Univ, Program Mol Med, Taipei, Taiwan

[4] Acad Sinica, Taipei, Taiwan

[5] Taichung Vet Gen Hosp, Div Urol, Dept Surg, Taichung, Taiwan

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

INFLAMED ADIPOSE-TISSUE; INSULIN SENSITIVITY; IL-4; OBESITY; METABOLISM; PATHOGENESIS; ASSOCIATION; EXPRESSION; RESISTANCE;

D O I：

10.1038/s41598-019-47908-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Accumulating evidence indicates that inflammation participates in the pathophysiological progress from insulin resistance, obesity, metabolic abnormalities, and type 2 diabetes mellitus. Our previous study reveals that interleukin-4 (IL-4) inhibits adipogenesis and promotes lipolysis to decrease lipid deposits by enhancing the activity of hormone sensitive lipase (HSL). The present study further dissects and characterizes the molecular mechanism of IL-4 in regulating HSL expression and lipolytic activity in the terminal differentiated 3T3-L1 mature adipocytes. Our results showed that IL-4 increased cAMP which then enhanced PKA activity and subsequent phosphorylation of HSL and perilipin. The phosphorylated HSL (p-HSL) translocated from cytoplasm to the surface of lipid droplets and exhibited lipolytic function. After being phosphorylated, p-perilipin also facilitated lipolysis through interacting with p-HSL. The in vitro findings were further verified by in vivo study in which IL-4 exhibited prolipolytic activity and enhanced HSL activity. In summary, the net outcome of IL-4 treatment is to reduce lipid storage by promoting lipolysis through enhancing HSL activity via cAMP/PKA pathway, the major route leading to lipolysis.

引用

页数：11

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