Characterization of the Drosophila melanogaster ribosomal proteome

被引：18

作者：

Alonso, Jana ^{[1
]}

Santaren, Juan F. ^{[1
]}

机构：

[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 08期

关键词：

Drosophila; ribosome; proteomics; 2D gels; MALDI-TOF;

D O I：

10.1021/pr0601483

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry to identifying proteins represented in a 2-D gel database of Drosophila melanogaster ribosomes. First, we purified ribosomes from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 58 Coomassie blue stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF. Using this strategy we identified 52 ribosomal spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF. The database can be used to analyze Minute mutants of Drosophila.

引用

页码：2025 / 2032

页数：8

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