Dstac Regulates Excitation-Contraction Coupling inDrosophilaBody Wall Muscles

被引：2

作者：

Hsu, I-Uen ^{[1
]}

Linsley, Jeremy W. ^{[1
,2
]}

Reid, Lilly E. ^{[1
]}

Hume, Richard I. ^{[1
]}

Leflein, Ari ^{[1
]}

Kuwada, John Y. ^{[1
,2
]}

机构：

[1] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA

来源：

FRONTIERS IN PHYSIOLOGY | 2020年 / 11卷

关键词：

stac adaptor protein; Drosophila; muscle; L-type voltage-gated calcium channel; excitation-contraction coupling; SKELETAL-MUSCLE; CALCIUM-CHANNEL; SARCOPLASMIC-RETICULUM; RYANODINE RECEPTOR; CHARGE MOVEMENT; CA2+ RELEASE; STAC3; SUBUNIT; GENE; EXPRESSION;

D O I：

10.3389/fphys.2020.573723

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Stac3 regulates excitation-contraction coupling (EC coupling) in vertebrate skeletal muscles by regulating the L-type voltage-gated calcium channel (Ca(v)channel). Recently astac-like gene,Dstac, was identified inDrosophilaand found to be expressed by both a subset of neurons and muscles. Here, we show that Dstac and Dmca1D, theDrosophilaL-type Ca(v)channel, are necessary for normal locomotion by larvae. Immunolabeling with specific antibodies against Dstac and Dmca1D found that Dstac and Dmca1D are expressed by larval body-wall muscles. Furthermore, Ca(2+)imaging of muscles of Dstac and Dmca1D deficient larvae found that Dstac and Dmca1D are required for excitation-contraction coupling. Finally, Dstac appears to be required for normal expression levels of Dmca1D in body-wall muscles. These results suggest that Dstac regulates Dmca1D during EC coupling and thus muscle contraction.

引用

页数：11

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