Immobilization of Fibrinogen Antibody on Self-Assembled Gold Monolayers for Immunosensor Applications

被引：6

作者：

Cho, Hongsik ^{[1
,2
,3
]}

Zook, Justin ^{[4
]}

Banner, Todd

Park, Sang-Hyug ^{[5
]}

Min, Byoung-Hyun ^{[6
,7
,8
]}

Hasty, Karen A. ^{[1
,2
,3
]}

Pinkhassik, Eugene

Lindner, Erno ^{[4
]}

机构：

[1] Univ Tennessee, Hlth Sci Ctr, Dept Orthpaed Surg & Biomed Engn, Memphis, TN 38104 USA

[2] Campbell Clinn, Dept Orthopaed Surg, Memphis, TN 38104 USA

[3] Vet Affairs Med Ctr, Memphis, TN 38104 USA

[4] Univ Memphis, Dept Biomed Engn, Memphis, TN 38152 USA

[5] Jungwon Univ, Dept Biomed Engn, Geosan 367700, South Korea

[6] Ajou Univ, Sch Med, Dept Orthopead Surg, Suwon 443749, South Korea

[7] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea

[8] Ajou Univ, Med Ctr, Cell Therapy Ctr, Suwon 443749, South Korea

来源：

TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2014年 / 11卷 / 01期

基金：

美国国家科学基金会;

关键词：

self-assembled monolayers (SAMs); anti-fibrinogen; immunosensors; enzyme-linked immuno sorbent assay (ELISA); ellipsometry; atomic force microscopy (AFM); gold monolayer; antibody immobilization; BIOSENSING APPLICATIONS; PLASMA-FIBRINOGEN; SURFACE; NANOTECHNOLOGY; PROTEINS;

D O I：

10.1007/s13770-013-1119-4

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.

引用

页码：10 / 15

页数：6

共 22 条

[1] Antibody-Conjugated Nanoparticles for Biomedical Applications [J].