A WD-FYVE protein binds to the kinases Akt and PKCζ/λ

被引：31

作者：

Fritzius, Thorsten ^{[1
]}

Burkard, Gabriela ^{[1
]}

Haas, Elvira ^{[1
]}

Heinrich, Jochen ^{[1
]}

Schweneker, Marc ^{[1
]}

Bosse, Magnus ^{[1
]}

Zimmermann, Sven ^{[1
]}

Frey, Alexander D. ^{[1
]}

Caelers, Antje ^{[1
]}

Bachmann, Andre S. ^{[1
]}

Moelling, Karin ^{[1
]}

机构：

[1] Univ Zurich, Inst Med Virol, CH-8006 Zurich, Switzerland

来源：

BIOCHEMICAL JOURNAL | 2006年 / 399卷 / 01期

关键词：

Akt; PC12; cell; FYVE domain; 3T3-L1; adipocyte; vesicle; WD-repeat;

D O I：

10.1042/BJ20060511

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

WD (tryptophan-aspartic acid dipeptide)-repeat proteins play a central role in signal transduction cascades by co-ordinating the interaction of key signalling molecules. We identified a novel propeller-FYVE [domain identified in Fab1p, YOTB, Vac1p and EEA1 (early endosome antigen 1)] protein, ProF, which is expressed in various cell lines and tissues and consists of seven WD-repeats and a FYVE domain. WD-repeat proteins offer a platform for protein-protein interactions by folding into a seven-bladed propeller-like structure, while the FYVE domain binds to phosphatidylinositol 3-phosphate present mainly on intracellular membranes. The ProF protein partially co-localizes with EEA1 on vesicular structures and binds to the protein kinases Akt and PKC zeta/lambda (protein kinase C zeta/lambda) via its WD-repeat propeller. ProF interacts more strongly with the kinases after hormonal stimulation. Endogenously expressed ProF and the two kinases interact in brain and in the preadipocyte cell line 3T3-L1, suggesting a role in secretory vesicular processes. In summary, we describe a new binding partner for kinases, located on vesicular structures in specialized cells, which may play a role for the spatial organization of signalling cascades.

引用

页码：9 / 20

页数：12

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