Recovery of solubilized δ-endotoxin from Bacillus thuringiensis subsp kurstaki fermentation broth

被引：0
|
作者：
Liu, BL [1 ]
Tzeng, YM [1 ]
Wei, CT [1 ]
机构：
[1] Natl Dong Hwa Univ, Inst Biotechnol, Shoufeng, Hualien, Taiwan
来源：
PEST MANAGEMENT SCIENCE | 2000年 / 56卷 / 05期
关键词：
Bacillus thuringiensis; delta-endotoxin; parasporal crystal; insecticidal activity;
D O I：
10.1002/(SICI)1526-4998(200005)56:5<448::AID-PS146>3.0.CO;2-O
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Insecticidal delta-endotoxin proteins, degraded from parasporal crystals by protease, were recovered by a simple procedure using heat treatment, solubilization, and ultrafiltration of a fermentation broth of Bacillus thuringiensis subsp kurstaki HD-1. A 68 kDa insecticidal protein was obtained and characterized by SDS-PAGE. The procedure described gave a nearly quantitative recovery of toxicity. Furthermore, bioassay results on larvae of the diamondback moth (Plutella xylostella) showed that the 68 kDa delta-endotoxin fraction (P1) was the principal insecticidal component to this target insect. A similar molecular mass polypeptide P2 (65 kDa) which was solubilized together with P1 from the parasporal crystals, gave relatively low mortality. (C) 2000 Society of Chemical Industry.
引用
收藏
页码：448 / 454
页数：7
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