Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria

被引：97

作者：

Geddes, Barney A. ^{[1
]}

Paramasivan, Ponraj ^{[2
]}

Joffrin, Amelie ^{[3
]}

Thompson, Amber L. ^{[3
]}

Christensen, Kirsten ^{[3
]}

Jorrin, Beatriz ^{[1
]}

Brett, Paul ^{[4
]}

Conway, Stuart J. ^{[3
]}

Oldroyd, Giles E. D. ^{[2
]}

Poole, Philip S. ^{[1
]}

机构：

[1] Univ Oxford, Dept Plant Sci, South Parks Rd, Oxford OX1 3RB, England

[2] Univ Cambridge, Sainsbury Lab, Bateman St, Cambridge CB2 1LR, England

[3] Univ Oxford, Dept Chem, Chem Res Lab, Mansfield Rd, Oxford OX1 3TA, England

[4] John Innes Ctr, Dept Metab Biol, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

BIOLOGICAL NITROGEN-FIXATION; RHIZOBIUM-MELILOTI; INOSITOL DEHYDROGENASE; MYOINOSITOL DEHYDROGENASE; ROOT MICROBIOTA; RHIZOPINE; CATABOLISM; CLONING; CHROMATOGRAPHY; PURIFICATION;

D O I：

10.1038/s41467-019-10882-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The root microbiota is critical for agricultural yield, with growth-promoting bacteria able to solubilise phosphate, produce plant growth hormones, antagonise pathogens and fix N-2. Plants control the microorganisms in their immediate environment and this is at least in part through direct selection, the immune system, and interactions with other microorganisms. Considering the importance of the root microbiota for crop yields it is attractive to artificially regulate this environment to optimise agricultural productivity. Towards this aim we express a synthetic pathway for the production of the rhizopine scyllo-inosamine in plants. We demonstrate the production of this bacterial derived signal in both Medicago truncatula and barley and show its perception by rhizosphere bacteria, containing bioluminescent and fluorescent biosensors. This study lays the groundwork for synthetic signalling networks between plants and bacteria, allowing the targeted regulation of bacterial gene expression in the rhizosphere for delivery of useful functions to plants.

引用

页数：11

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