Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration

被引:143
作者
Gan, Zhuo [1 ]
Ding, Liya [1 ,2 ]
Burckhardt, Christoph J. [1 ,2 ]
Lowery, Jason [4 ]
Zaritsky, Assaf [1 ]
Sitterley, Karlyndsay [2 ]
Mota, Andressa [2 ]
Costigliola, Nancy [2 ]
Starker, Colby G. [3 ]
Voytas, Daniel F. [3 ]
Tytell, Jessica [2 ]
Goldman, Robert D. [4 ]
Danuser, Gaudenz [1 ,2 ]
机构
[1] Univ Texas Southwestern Med Ctr Dallas, Dept Cell Biol, Dallas, TX 75235 USA
[2] Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA
[3] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55455 USA
基金
瑞士国家科学基金会;
关键词
CYTOSKELETAL CROSSTALK; DYNAMICS; ADHESION; ACTIN; ORGANIZATION; ARCHITECTURE; FIBROBLASTS; CONTRIBUTES; PROTEINS; DESIGN;
D O I
10.1016/j.cels.2016.08.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Increased expression of vimentin intermediate filaments (VIFs) enhances directed cell migration, but the mechanism behind VIFs' effect on motility is not understood. VIFs interact with microtubules, whose organization contributes to polarity maintenance in migrating cells. Here, we characterize the dynamic coordination of VIF and microtubule networks in wounded monolayers of retinal pigment epithelial cells. By genome editing, we fluorescently labeled endogenous vimentin and a-tubulin, and we developed computational image analysis to delineate architecture and interactions of the two networks. Our results show that VIFs assemble an ultrastructural copy of the previously polarized microtubule network. Because the VIF network is long-lived compared to the microtubule network, VIFs template future microtubule growth along previous microtubule tracks, thus providing a feedback mechanism that maintains cell polarity. VIF knockdown prevents cells from polarizing and migrating properly during wound healing. We suggest that VIFs' templating function establishes a memory in microtubule organization that enhances persistence in cell polarization in general and migration in particular.
引用
收藏
页码:252 / +
页数:20
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