Localized Deposition of Chitosan as Matrix for Enzyme Immobilization

被引：19

作者：

Chen, Po-Chung ^{[1
,2
]}

Chen, Richie L. C. ^{[2
]}

Cheng, Tzong-Jih ^{[2
]}

Wittstock, G. ^{[1
]}

机构：

[1] Carl von Ossietzky Univ Oldenburg, Dept Pure & Appl Chem, CIS, Fac Math & Sci, Oldenburg, Germany

[2] Natl Taiwan Univ, Dept Bioind Mechatron Engn, Taipei, Taiwan

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 07期

关键词：

Chitosan; pH Gradient; Localized electrodeposition; Scanning electrochemical microscopy; Microfluidic channels; SCANNING ELECTROCHEMICAL MICROSCOPY; SELF-ASSEMBLED MONOLAYERS; POLYMER-FILMS; PATTERNED SURFACE; SELECTIVE PROTEIN; SOFT LITHOGRAPHY; GOLD; ELECTRODEPOSITION; CELLS; MICROELECTRODES;

D O I：

10.1002/elan.200804514

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A local electrodeposition method was developed for chitosan by exploiting a pH gradient between a macroscopic electrode (the support) and a much smaller counter electrode. The deposition was confined either by using the direct mode of scanning electrochemical microscopy (SECM) or by performing the deposition in channels of a microfluidic network. The roughness was characterized by noncontact scanning force microscopy. The availability of amino groups at the surface of the microstructures was visualized after labeling by confocal laser scanning microscopy. The enzyme glucose oxidase could be entrapped during the electrochemical deposition and showed activity as seen by SECM images.

引用

页码：804 / 810

页数：7

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