CTL induction by DNA vaccine with Toxoplasma gondii-HSP70 gene

被引：21

作者：

Chu, Deyong ^{[1
]}

Moroda, Masataka ^{[1
]}

Piao, Lian-Xun ^{[1
]}

Aosai, Fumie ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Infect & Host Def, Chuo Ku, Chiba 2608670, Japan

来源：

PARASITOLOGY INTERNATIONAL | 2014年 / 63卷 / 02期

基金：

日本学术振兴会;

关键词：

Toxoplasma gondii; T. gondii HSP70; DC vaccine; Cytotoxic T lymphocytes (CTL); TOXOPLASMA-GONDII-INFECTION; CD8+ T-CELLS; LETHAL ANAPHYLACTIC REACTION; DENDRITIC CELLS; PROTECTIVE IMMUNITY; IFN-GAMMA; MICE; ANTIGEN; RESPONSES; CD4(+);

D O I：

10.1016/j.parint.2014.01.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) is a tachyzoite-specific virulent molecule. The DNA vaccine with T.g.HSP70 gene targeting peripheral epidermal or dermal dendritic cells (DC) induces in vivo DC maturation and successive early Th1 polarization at the draining lymph nodes (dLN) of C57BL/6 mice. In the present study, induction of cytotoxic T lymphocytes (CTL) has been explored. The CTL specific for a syngeneic DC line, DC2.4, either transfected with T. g.HSP70 gene or pulsed with recombinant T. g.HSP70 are induced in the spleen of the vaccinated mice. This CTL lyses T. gondii-infected, but not uninfected, DC2A. Both CD8(+) and CD4(+) CTL are induced by the vaccine, and Fas/Fas ligand-mediated cytolysis dominantly participates in their CTL activities. Adoptive transfer experiments reveal that the vaccine-induced CD8(+) or CD4(+) T cells possess a protective role for toxoplasmosis at both acute and chronic phases of infection. (c) 2014 Published by Elsevier Ireland Ltd.

引用

页码：408 / 416

页数：9

共 53 条

[31] Kinetics in parasite abundance in susceptible and resistant mice infected with an avirulent strain of Toxoplasma gondii by using quantitative competitive PCR [J].

Luo, WT ;

Aosai, F ;

Ueda, M ;

Yamashita, K ;

Shimizu, K ;

Sekiya, S ;

Yano, A .

JOURNAL OF PARASITOLOGY, 1997, 83 (06) :1070-1074

[32] Innate immunity in DNA vaccine with Toxoplasma gondii-heat shock protein 70 gene that induces DC activation and Th1 polarization [J].

Makino, Masayuki ;

Uemura, Noriko ;

Moroda, Masataka ;

Kikumura, Akitoshi ;

Piao, Lian-Xun ;

Mohamed, Rabie M. ;

Aosai, Fumie .

VACCINE, 2011, 29 (10) :1899-1905

[33] Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes [J].

Mohamed, RM ;

Aosai, F ;

Chen, M ;

Mun, HS ;

Norose, K ;

Belal, US ;

Piao, LX ;

Yano, A .

VACCINE, 2003, 21 (21-22) :2852-2861

[34] Human CD4(+) and CD8(+) T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells [J].

Montoya, JG ;

Lowe, KE ;

Clayberger, C ;

Moody, D ;

Do, D ;

Remington, JS ;

Talib, S ;

Subauste, CS .

INFECTION AND IMMUNITY, 1996, 64 (01) :176-181

[35]

Mun HS, 2000, CELL STRESS CHAPERON, V5, P328, DOI 10.1379/1466-1268(2000)005<0328:TGHAAD>2.0.CO

[36]

[37] Role of Toxoplasma gondii HSP70 and Toxoplasma gondii HSP30/bag1 in antibody formation and prophylactic immunity in mice experimentally infected with Toxoplasma gondii [J].

Mun, HS ;

Aosai, F ;

Yano, A .

MICROBIOLOGY AND IMMUNOLOGY, 1999, 43 (05) :471-479

[38] A novel B-2 suppressor cell regulating susceptibility/resistance of mice to Toxoplasma gondii infection [J].

Mun, HS ;

Aosai, F ;

Fang, H ;

Piao, LX ;

Winn, T ;

Norose, K ;

Yano, A .

MICROBIOLOGY AND IMMUNOLOGY, 2005, 49 (09) :853-858

[39] CD8+ T-CELLS ARE THE MAJOR LYMPHOCYTE SUBPOPULATION INVOLVED IN THE PROTECTIVE IMMUNE-RESPONSE TO TOXOPLASMA-GONDII IN MICE [J].

PARKER, SJ ;

ROBERTS, CW ;

ALEXANDER, J .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1991, 84 (02) :207-212

[40] A NOVEL SERINE ESTERASE EXPRESSED BY CYTO-TOXIC LYMPHOCYTES-T [J].

PASTERNACK, MS ;

EISEN, HN .

NATURE, 1985, 314 (6013) :743-745

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