Mueller matrix signature in advanced fluorescence microscopy imaging

被引：16

作者：

Mazumder, Nirmal ^{[1
,2
]}

Qiu, Jianjun ^{[1
,3
]}

Kao, Fu-Jen ^{[1
]}

Diaspro, Alberto ^{[4
]}

机构：

[1] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan

[2] Manipal Univ, Sch Life Sci, Dept Biophys, Manipal 576104, Karnataka, India

[3] Huazhong Univ Sci & Technol, Key Lab Biomed Photon, Wuhan, Hubei, Peoples R China

[4] Ist Italiano Tecnol, Nanoscopy, Via Morego 30, I-16163 Genoa, Italy

来源：

JOURNAL OF OPTICS | 2017年 / 19卷 / 02期

关键词：

microscopy; polarimetric imaging; fluorescence microscopy; imaging systems; 2ND-HARMONIC GENERATION MICROSCOPY; MOLECULAR-STRUCTURE; TURBID MEDIA; POLARIZATION; SCATTERING; LIGHT; POLARIMETRY; DECOMPOSITION; SPECTROSCOPY; PARAMETERS;

D O I：

10.1088/2040-8986/aa5114

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.

引用

页数：6

共 32 条

[1]

[Anonymous], 1957, Light scattering by small particles

[2] Mueller matrices in fluorescence scattering [J].