Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels

被引：113

作者：

Bao, Yan-Yuan ^{[1
]}

Tang, Xu-Dong ^{[1
]}

Lv, Zu-Yao ^{[1
]}

Wang, Xiu-Ye ^{[2
]}

Tian, Cai-Hong ^{[1
]}

Xu, Yi Peng ^{[1
]}

Zhang, Chuan-Xi ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310029, Zhejiang, Peoples R China

[2] Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China

来源：

GENOMICS | 2009年 / 94卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Suppression subtractive hybridization; BmNPV; Bornbyx mori; Antiviral response; IN-VITRO REPLICATION; SIGNALING PATHWAYS; ANTIVIRAL ACTIVITY; INFECTION; SILKWORM; PROTEIN; VIRUS; CDNA; HEMOLYMPH; IDENTIFICATION;

D O I：

10.1016/j.ygeno.2009.04.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated variations in the gene expression of Bombyx mori following infection with a nucleopolyhedrovirus (BmNPV). Two B. mori strains, KN and 306, which are highly resistant and susceptible to BmNPV infection, respectively, were used in this Study. The infection profiles of BmNPV in the B. mori KN and 306 larvae revealed that the virus invaded the midguts of both these strains. However, its proliferation was notably inhibited in the midgut of the resistant strain. By using the suppression subtractive hybridization method, two cDNA libraries were constructed in order to compare the BmNPV responsive gene expressions between the two silkworm lines. In total, 62 differentially expressed genes were obtained. Real-time qPCR analysis confirmed that eight genes were significantly up-regulated in the midgut of the M strain following BmNPV infection. Our results imply that these up-regulated genes may be involved in the B. mori immune response against BmNPV infection. (C) 2009 Elsevier Inc. All rights reserved.

引用

收藏

页码：138 / 145

页数：8

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