Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae

被引：43
|
作者：
Gubala, Aneta J. [1 ]
Proll, David F. [1 ]
机构：
[1] Def Sci & Technol Org, Human Protect & Performance Div, Melbourne, Vic, Australia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 09期
关键词：
D O I：
10.1128/AEM.02597-05
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.
引用
收藏
页码：6424 / 6428
页数：5
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