Impedimetric graphene-based biosensor for the detection of Escherichia coli DNA

被引：26

作者：

Zainudin, Nurulasma ^{[1
]}

Hairul, Ab. Rahim Mohd ^{[1
]}

Yusoff, Mashitah Mohd ^{[1
]}

Tan, Ling Ling ^{[2
]}

Chong, Kwok Feng ^{[1
]}

机构：

[1] Univ Malaysia Pahang, Fac Ind Sci & Technol, Kuantan 26300, Pahang, Malaysia

[2] Univ Kebangsaan Malaysia, SEADPRI, LESTARI, Ukm Bangi 43600, Selangor, Malaysia

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 19期

关键词：

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; IMMUNOMAGNETIC SEPARATION; HYBRIDIZATION; OXIDE; IMMUNOASSAY; MODULATION; BACTERIA; CRYSTAL; O157/H7; BINDING;

D O I：

10.1039/c4ay01836b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free impedimetric DNA biosensor based on graphene nanosheets has been developed for the detection of Escherichia coli O157:H7 strain GZ-021210. Probe DNA (pDNA) of E. coli was immobilized onto graphene nanosheets by the surface functionalization of graphene with 1-pyrenebutyric acid (PyBA) followed by carbodiimide linkage. The hybridization of complementary DNA (cDNA) of E. coli with the immobilized pDNA increased the electron transfer resistance of the graphene nanosheets, as observed by electrochemical impedance spectroscopy (EIS). The E. coli DNA biosensor displayed a wide range of linear response (1.0 x 10(-10) M to 1.0 x 10(-14) M), low detection limit (0.7 x 10(-15) M), single-base mismatch selectivity, high robustness and good reproducibility. The current work demonstrates an important advancement in the development of a sensitive biosensor for E. coli detection.

引用

页码：7935 / 7941

页数：7

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