Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils

被引：12

作者：

Ahrentorp, Fredrik ^{[1
]}

Blomgren, Jakob ^{[1
]}

Jonasson, Christian ^{[1
]}

Sarwe, Anna ^{[1
]}

Sepehri, Sobhan ^{[2
]}

Eriksson, Emil ^{[2
]}

Kalaboukhov, Alexei ^{[2
]}

Jesorka, Aldo ^{[2
]}

Winkler, Dag ^{[2
]}

Schneiderman, Justin F. ^{[3
,4
]}

Nilsson, Mats ^{[5
]}

Albert, Jan ^{[6
,7
]}

de la Torre, Teresa Zardan Gomez ^{[8
]}

Stromme, Maria ^{[8
]}

Johansson, Christer ^{[1
]}

机构：

[1] Acreo Swedish ICT AB, Arvid Hedvalls Backe 4, Gothenburg, Sweden

[2] Chalmers, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden

[3] Univ Gothenburg, Inst Neurosci & Physiol, Gothenburg, Sweden

[4] MedTech West, Gothenburg, Sweden

[5] Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden

[6] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

[7] Karolinska Univ Hosp, Dept Clin Microbiol, Stockholm, Sweden

[8] Uppsala Univ, Uppsala, Sweden

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2017年 / 427卷

关键词：

Magnetic nanoparticles; Magnetic multi-core particles; AC susceptometry; RCA coil; Differential induction coil; Biodetection; PARTICLES; DIAGNOSTICS; NANOBEADS; SENSORS;

D O I：

10.1016/j.jmmm.2016.10.041

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 mu m. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 mu L) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

引用

页码：14 / 18

页数：5

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