Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

被引：16

作者：

Cui, Fengjie ^{[2
]}

Li, Yin ^{[1
]}

Liu, Zhiqiang ^{[3
,4
]}

Zhao, Hui ^{[5
]}

Ping, Lifeng ^{[3
,6
]}

Ping, Liying

Yang, Yinan ^{[7
]}

Xue, Yaping ^{[3
]}

Yan, Lijiao ^{[8
]}

机构：

[1] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA

[2] Jiangsu Univ, Sch Food & Biotechnol, Zhenjiang 2120013, Peoples R China

[3] Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China

[4] NYU, Polytech Inst, Dept Chem & Biol Sci,Metrotech Ctr 6, Ctr Biocatalysis & Bioproc Macromol,NSF I UCRC, Brooklyn, NY 11201 USA

[5] N Dakota State Univ, Dept Stat, Fargo, ND 58105 USA

[6] Zhejiang Acad Agr Sci, Inst Qual & Standard Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

[7] Jilin Agr Univ, Coll Hort, Changchun 130118, Peoples R China

[8] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2009年 / 25卷 / 04期

关键词：

Identification; Penicillium thiersii ZH-19; Response surface methodology; Optimization; Xylanase; IDENTIFICATION; ARABINOXYLANS;

D O I：

10.1007/s11274-008-9942-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8A degrees C, xylan 13.2 g l(-1) and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml(-1). Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL(-1) in the flask experiments and 80.23 U mL(-1) in the scale of 15-L fermenter under the optimal condition.

引用

页码：721 / 725

页数：5

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